Candidatus Scalindua, a Biological Solution to Treat Saline Recirculating Aquaculture System Wastewater
Candidatus Scalindua, a Biological Solution to Treat Saline Recirculating Aquaculture System Wastewater
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DOI：
10.3390/pr11030690
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发表时间：
2023-02
期刊：
影响因子：
3.5
通讯作者：
F. Micolucci;Jonathan A. C. Roques;Geoffrey S. Ziccardi;N. Fujii;K. Sundell;T. Kindaichi
中科院分区：
文献类型：
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作者：
F. Micolucci;Jonathan A. C. Roques;Geoffrey S. Ziccardi;N. Fujii;K. Sundell;T. Kindaichi
循环水养殖系统（RAS）是水产养殖业可持续发展的一种有前途的模式。RAS目前的一个限制是含氮废物，铵（NH4+），亚硝酸盐（NO2−）和硝酸盐（NO3−）的产生和潜在积累，这可能会影响鱼类的健康和福利。在以前的实验中，我们已经证明，海洋厌氧氨氧化细菌Escheridatus Scalindua是一个有前途的候选人，以处理海洋，冷水RAS的废水（WW）。然而，细菌的活性在直接暴露于RAS WW后受到负面影响。在本研究中，我们进一步研究了Ca的潜力。Scalindua在三阶段实验中治疗海洋RAS WW。在第一阶段（对照，83天），Ca. Scalindua喂食合成饲料，富含NH4+，NO2−和微量元素（TE）混合物。NH4+和NO2−的去除率分别达到98.9%和99.6%。在第二阶段（116天），我们逐渐增加钙的暴露。Scalindua到富含氮的RAS WW的时间约为80天。在最后阶段（79天），我们调查了TE补充钙的需求。Scalindua完全适应100%RAS WW后。我们的研究结果表明，逐渐暴露的Ca。Scalindua导致成功适应100%RAS WW，在整个实验过程中保持高的NH4+和NO2−去除率。尽管相对丰度略有下降（从21.4%到16.7%），Ca。在整个实验过程中，Scalindua仍然是颗粒中的优势种。我们的结论是，Ca。Scalindua可以成功地用于处理海洋RAS WW，而不添加TE，一旦给予足够的时间来适应其新的基质。未来的研究需要确定Ca对最佳RAS WW治疗的具体需求。中试规模的Scalindua。
Recirculating aquaculture systems (RAS) are promising candidates for the sustainable development of the aquaculture industry. A current limitation of RAS is the production and potential accumulation of nitrogenous wastes, ammonium (NH4+), nitrite (NO2−) and nitrate (NO3−), which could affect fish health and welfare. In a previous experiment, we have demonstrated that the marine anammox bacteria Candidatus Scalindua was a promising candidate to treat the wastewater (WW) of marine, cold-water RAS. However, the activity of the bacteria was negatively impacted after a direct exposure to RAS WW. In the current study, we have further investigated the potential of Ca. Scalindua to treat marine RAS WW in a three-phase experiment. In the first phase (control, 83 days), Ca. Scalindua was fed a synthetic feed, enriched in NH4+, NO2− and trace element (TE) mix. Removal rates of 98.9% and 99.6% for NH4+ and NO2−, respectively, were achieved. In the second phase (116 days), we gradually increased the exposure of Ca. Scalindua to nitrogen-enriched RAS WW over a period of about 80 days. In the last phase (79 days), we investigated the needs of TE supplementation for the Ca. Scalindua after they were fully acclimated to 100% RAS WW. Our results show that the gradual exposure of Ca. Scalindua resulted in a successful acclimation to 100% RAS WW, with maintained high removal rates of both NH4+ and NO2− throughout the experiment. Despite a slight decrease in relative abundance (from 21.4% to 16.7%), Ca. Scalindua remained the dominant species in the granules throughout the whole experiment. We conclude that Ca. Scalindua can be successfully used to treat marine RAS WW, without the addition of TE, once given enough time to acclimate to its new substrate. Future studies need to determine the specific needs for optimal RAS WW treatment by Ca. Scalindua at pilot scale.