Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus Attachment Patterns on Glass Surfaces with Nanoscale Roughness
Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus Attachment Patterns on Glass Surfaces with Nanoscale Roughness
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DOI：
10.1007/s00284-008-9320-8
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发表时间：
2009-03-01
影响因子：
2.6
通讯作者：
Ivanova, Elena P.
中科院分区：
文献类型：
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作者：
Mitik-Dineva, Natasa;Wang, James;Ivanova, Elena P.
研究了大肠杆菌K12、铜绿假单胞菌ATCC 9027和金黄色葡萄球菌CIP 68.5在不同纳米级粗糙度玻璃表面的附着倾向。采用接触角和表面电荷测量、原子力显微镜（AFM）、扫描电子显微镜（SEM）和共聚焦激光扫描显微镜（CLSM）来表征基质和细菌表面。对玻璃表面的修饰导致表面形貌发生纳米级的变化，而表面的物理化学特性几乎保持不变。AFM分析表明，整体表面粗糙度参数降低了60-70%。SEM、CLSM和AFM分析清楚地表明，尽管大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌呈现出明显不同的附着模式，但所有物种都表现出更大的对“纳米光滑”表面的粘附倾向。细菌对表面修饰的反应是细胞代谢活性的显著变化，表现为细胞形态的特征，细胞外聚合物物质的产生以及进行附着的细菌细胞数量的增加。
Attachment tendencies of Escherichia coli K12, Pseudomonas aeruginosa ATCC 9027, and Staphylococcus aureus CIP 68.5 onto glass surfaces of different degrees of nanometer-scale roughness have been studied. Contact-angle and surface-charge measurements, atomic force microscopy (AFM), scanning electron microscopy (SEM), and confocal laser scanning microscopy (CLSM) were employed to characterize substrata and bacterial surfaces. Modification of the glass surface resulted in nanometer-scale changes in the surface topography, whereas the physicochemical characteristics of the surfaces remained almost constant. AFM analysis indicated that the overall surface roughness parameters were reduced by 60-70%. SEM, CLSM, and AFM analysis clearly demonstrates that although E. coli, P. aeruginosa and S. aureus present significantly different patterns of attachment, all of the species exhibited a greater propensity for adhesion to the "nano-smooth" surface. The bacteria responded to the surface modification with a remarkable change in cellular metabolic activity, as shown by the characteristic cell morphologies, production of extracellular polymeric substances, and an increase in the number of bacterial cells undergoing attachment.