M-ds-P21 induces cell apoptosis in bladder cancer T24 cells through P53 independent pathway
M-ds-P21 induces cell apoptosis in bladder cancer T24 cells through P53 independent pathway
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M-ds-P21 通过 P53 独立途径诱导膀胱癌 T24 细胞凋亡。
DOI：
10.4103/0973-1482.110367
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发表时间：
2013-01-01
影响因子：
1.3
通讯作者：
Guo, Yinglu
中科院分区：
文献类型：
--
作者：
Wang, Haifeng;Liu, Wujiang;Guo, Yinglu
目的：探讨M-DS-P21对膀胱癌T24细胞凋亡的影响及其可能机制。材料与方法：采用细胞形态和Western印迹法检测M-DS-P21对T24细胞的影响。Annexin-V流式细胞仪分析细胞凋亡率。为了揭示P53在M-DS-P21诱导T24细胞凋亡中的作用，我们在用M-DS-P21处理T24细胞之前先敲除P53，然后用Western印迹法检测P21和凋亡相关蛋白的表达。结果：M-DS-P21和DS-P21作用3天后细胞形态均发生改变，导致细胞发生凋亡。M-ds-P21和ds-P21诱导的细胞凋亡不依赖于P53，而依赖于caspase。与DS-P21相比，M-DS-P21显著提高了DS-RNA在T24细胞中的生物利用度。结论：与DS-P21相比，M-DS-P21可诱导更多的细胞凋亡。M-ds-P21作用增强的机制部分是由于DS-RNA在人膀胱癌T24细胞中的生物利用度提高，而不是P53依赖的，而是caspase依赖的。
Objectives: To investigate the effect of M-ds-P21 on the apoptosis of bladder cancer T24 cells and its potential mechanism.Materials and Methods: Effect of M-ds-P21 on T24 cells were assessed by cell morphology and Western blot. Apoptosis was quantified by Annexin-V flow-cytometry analysis. To uncover the role of P53 in M-ds-P21-mediated apoptosis of T24 cells, we knocked down P53 before treating cells with M-ds-P21, and then assayed P21 and apoptosis-related protein by Western blot. To uncover the mechanism by which M-ds-P21 played stronger effect than ds-P21, we performed confocal microscope analyses.Results: Both M-ds-P21 and ds-P21 treatment changed the cell morphology, leading to cell apoptosis after 3 days. Apoptosis induced by M-ds-P21 and ds-P21 treatment is not P53-dependent but caspase-dependent. Compared with ds-P21, M-ds-P21 significantly increased the bioavailability of ds-RNA in T24 cells.Conclusions: M-ds-P21 treatment induces more apoptotic population than ds-P21 does. The mechanism for stronger effect of M-ds-P21 is partly due to the enhanced bioavailability of ds-RNA in human bladder cancer T24 cells, and not P53-dependent but caspase-dependent.