Endogenous small RNAs in grain: Semi-quantification and sequence homology to human and animal genes
Endogenous small RNAs in grain: Semi-quantification and sequence homology to human and animal genes
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DOI：
10.1016/j.fct.2008.11.025
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发表时间：
2009-02-01
影响因子：
4.3
通讯作者：
Roberts, James K.
中科院分区：
文献类型：
--
作者：
Ivashuta, Sergey I.;Petrick, Jay S.;Roberts, James K.
小干扰RNA （sirna）和微RNA （miRNAs）是RNA干扰（RNAi）的效应分子，RNAi是植物、哺乳动物和其他真核生物中高度保守的基于RNA的基因抑制机制。内源性rnai为基础的基因抑制已利用自然和通过传统育种实现所需的植物表型。目前的研究表明，内源性小rna，如sirna和miRNAs，在大豆种子、玉米籽粒和水稻等传统上用于食品和饲料的植物组织中含量丰富。许多外生植物小rna被发现与人类和其他哺乳动物的基因具有完全的互补性。从安全食用的食品和饲料作物（如大豆、玉米和水稻）中提取的谷物中富含外源性小RNA分子，以及这些膳食小RNA与人类和动物基因组和转录组的同源性，确立了膳食小RNA安全食用的历史。(C) 2008 Elsevier Ltd版权所有。
Small interfering RNAs (siRNAs) and microRNAs (miRNAs) are effector molecules of RNA interference (RNAi), a highly conserved RNA-based gene suppression mechanism in plants, mammals and other eukaryotes. Endogenous RNAi-based gene suppression has been harnessed naturally and through conventional breeding to achieve desired plant phenotypes. The present study demonstrates that endogenous small RNAs, such as siRNAs and miRNAs, are abundant in soybean seeds, corn kernels, and rice grain, plant tissues that are traditionally used for food and feed. Numerous enclogenous plant small RNAs were found to have perfect complementarity to human genes as well as those of other mammals. The abundance of enclogenous small RNA molecules in grain from safely consumed food and feed crops such as soybean, corn, and rice and the homology of a number of these dietary small RNAs to human and animal genomes and transcriptomes establishes a history of safe consumption for dietary small RNAs. (C) 2008 Elsevier Ltd. All rights reserved.