N-(3-oxo-hexanoyl)-homoserine lactone has a critical contribution to the quorum-sensing-dependent regulation in phytopathogen Pseudomonas syringae pv. tabaci 11528
N-(3-oxo-hexanoyl)-homoserine lactone has a critical contribution to the quorum-sensing-dependent regulation in phytopathogen Pseudomonas syringae pv. tabaci 11528
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N-(3-氧代-己酰基)-高丝氨酸内酯对植物病原体丁香假单胞菌的群体感应依赖性调节具有重要作用。
DOI：
10.1093/femsle/fnw265
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发表时间：
2016-12-01
影响因子：
2.1
通讯作者：
Zhuang, Guoqiang
中科院分区：
文献类型：
--
作者：
Cheng, Feifei;Ma, Anzhou;Zhuang, Guoqiang
植物病原菌假单胞菌（Pseudomonasacrylingae pv.）在大豆和烟草植物中引起野火病的烟粉虱11528（P. tabingae 11528）处理PsyI-PsyR群体感应（QS）系统，其中PsyI是N-（3-氧代-己酰基）-高丝氨酸内酯（3 OC 6-HSL）合酶。与P. eriningae 11528 AHL缺陷突变体相比，在从指数期到稳定期的过渡期，在用外源3 OC 6-HSL生长的AHL缺陷突变体中使用RNA测序（RNA-seq）鉴定了845个3 OC 6-HSL依赖性基因，并且其中许多与毒力相关，其被负调控。对这些基因的基因本体和KEGG途径富集分析表明，最显著的调控涉及细菌运动。此外，与AHL缺陷型突变体相比，在野生型和AHL缺陷型突变体中观察到类似的基因表达谱在生长阶段与外源3 OC 6-HSL。这些结果表明，3 OC 6-HSL对QS依赖的基因表达调控有重要贡献，3 OC 6-HSL依赖的调控可能在植物感染中起重要作用。
The phytopathogen Pseudomonas syringae pv. tabaci 11528 (P. syringae 11528), causing wild-fire disease in soybean and tobacco plants, processes PsyI-PsyR quorum-sensing (QS) system, in which PsyI is the N-(3-oxo-hexanoyl)-homoserine lactone (3OC6-HSL) synthase. In comparison to P. syringae 11528 AHL-deficient mutant, 845 3OC6-HSL-dependent genes were identified using RNA sequencing (RNA-seq) in the AHL-deficient mutant grown with exogenous 3OC6-HSL in the transition from the exponential to the stationary phase, and many of them were associated with virulence, which were negatively regulated. The gene ontology and KEGG pathway enrichment analysis of those genes presented that the most pronounced regulation was involved in bacterial motility. Moreover, similar expression profiles of genes during growth phases were observed in both the wild type and the AHL-deficient mutant with exogenous 3OC6-HSL compared with the AHL-deficient mutant. These findings imply that 3OC6-HSL has a critical contribution to the QS-dependent regulation on gene expression, and 3OC6-HSL-dependent regulation may play a significant role in plant infection.