Zfp57 mutant ES cell lines directly derived from blastocysts.
Zfp57 mutant ES cell lines directly derived from blastocysts.
复制标题
DOI：
10.1016/j.scr.2015.12.038
复制
发表时间：
2016-03
影响因子：
1.2
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Lau HT;Liu L;Li X
zfp57是小鼠胚胎基因组印记的主要调节因子。为了进一步测试其功能，我们直接从小鼠囊胚中衍生出多个Zfp57突变ES克隆。事实上，我们发现在这些Zfp57突变ES克隆中，DNA甲基化印记在大多数检测的印记控制区域丢失，与先前研究中在Zfp57突变胚胎中观察到的相似。该结果表明，这些囊胚来源的Zfp57突变ES克隆可用于Zfp57在基因组印迹中的功能分析。
Zfp57 is a master regulator of genomic imprinting in mouse embryos. To further test its functions, we have derived multiple Zfp57 mutant ES clones directly from mouse blastocysts. Indeed, we found DNA methylation imprint was lost at most examined imprinting control regions in these Zfp57 mutant ES clones, similar to what was observed in Zfp57 mutant embryos in the previous studies. This result indicates that these blastocyst-derived Zfp57 mutant ES clones can be employed for functional analyses of Zfp57 in genomic imprinting.