Fibronectin-mediated endothelialisation of chitosan porous matrices
Fibronectin-mediated endothelialisation of chitosan porous matrices
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DOI：
10.1016/j.biomaterials.2009.06.056
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发表时间：
2009-10-01
期刊：
影响因子：
14
通讯作者：
Kirkpatrick, C. J.
中科院分区：
文献类型：
--
作者：
Amaral, Isabel F.;Unger, Ronald E.;Kirkpatrick, C. J.
研究了壳聚糖（Ch）多孔基质被人微血管内皮细胞（HPMEC-ST 1. 6R细胞系）和大血管内皮细胞（即HUVEC）定殖的能力。具体地说，我们评估了如果以前的孵育Ch在纤连蛋白（FN）的解决方案是有效的促进内皮细胞（EC）粘附Ch矩阵与不同程度的乙酰化（DA）。在FN物理吸附后，发现两种DA之间存在显著差异，即DA 4%和15%。虽然细胞粘附受损Ch DA 15%，EC不仅能够坚持Ch DA 4%，但也蔓延和殖民的支架，保留EC表型和血管生成的潜力。为了解释两种DA之间观察到的差异，使用I-125-FN和FN细胞结合域的免疫荧光标记进行了蛋白质吸附研究。与发现的较高细胞数一致，DA为4%的支架显示出较高数量的暴露的FN细胞结合结构域以及在竞争性蛋白存在下更大的吸附FN和保留和交换吸附的FN的能力。这些研究结果表明，DA是一个关键参数调节EC粘附FN包被Ch通过影响吸附的蛋白质层。(C)2009爱思唯尔有限公司版权所有。
Chitosan (Ch) porous matrices were investigated regarding their ability to be colonized by human microvascular endothelial cells (HPMEC-ST1.6R cell line) and macrovascular endothelial cells namely HUVECs. Specifically we assessed if previous incubation of Ch in a fibronectin (FN) solution was effective in promoting endothelial cell (EC) adhesion to Ch matrices with different degrees of acetylation (DAs). Upon FN physiadsorption, marked differences were found between the two DAs investigated, namely DA 4% and 15%. While cell adhesion was impaired on Ch with DA 15%, ECs were able to not only adhere to Ch with DA 4%, but also to spread and colonize the scaffolds, with retention of the EC phenotype and angiogenic potential. To explain the observed differences between the two DAs, protein adsorption studies using I-125-FN and immunofluorescent labelling of FN cell-binding domains were carried out. in agreement with the higher cell numbers found, scaffolds with DA 4% revealed a higher number of exposed FN cell-binding domains as well as greater ability to adsorb FN and to retain and exchange adsorbed FN in the presence of competitive proteins. These findings suggest that the DA is a key parameter modulating EC adhesion to FN-coated Ch by influencing the adsorbed protein layer. (C) 2009 Elsevier Ltd. All rights reserved.