Construction of a 350-kb sequence-ready 11q13 cosmid contig encompassing the markers D11S4933 and D11S546: Mapping of 11 genes and 3 tumor-associated translocation breakpoints
Construction of a 350-kb sequence-ready 11q13 cosmid contig encompassing the markers D11S4933 and D11S546: Mapping of 11 genes and 3 tumor-associated translocation breakpoints
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DOI：
10.1006/geno.2000.6194
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发表时间：
2000-05-15
期刊：
影响因子：
4.4
通讯作者：
van Kessel, AG
中科院分区：
文献类型：
--
作者：
van Asseldonk, M;Schepens, M;van Kessel, AG
在此之前，我们在染色体11 q13区域的标记D11 S4933和D11 S546之间定位了三个新的人类肿瘤相关易位断点。为了便于这些断点的分子分析，我们构建了一个连续的序列准备粘粒和PAC重叠群约350 kb，包括标记D11 S4933和D11 S546。此外，还生成了详细的转录本图谱。这导致了重叠群中11个基因和EST的精确定位，其中包括4个已知定位在11 q13区域的基因。我们定位在重叠群中的其他三个基因显示出与来自人类和/或其他物种的未定位基因的同源性。三个EST是新的。部分粘粒测序导致在几个报告的基因的转录方向的建立。该重叠群将有助于详细描述肿瘤相关染色体断裂点和鉴定其他11 q13相关疾病基因。(C)北京大学出版社.
Previously, we located three novel human tumor-associated translocation breakpoints in the chromosome 11q13 region between the markers D11S4933 and D11S546. To facilitate the molecular analysis of these breakpoints, we have constructed a continuous sequence-ready cosmid and PAC contig of approximately 350 kb, including the markers D11S4933 and D11S546. In addition, a detailed transcript map was generated. This resulted in the precise positioning of 11 genes and ESTs within the contig, including 4 genes already known to map in the 11q13 region. Three other genes that we positioned within the contig showed homologies to unmapped genes from human and/or other species. Three ESTs were novel. Partial cosmid sequencing resulted in the establishment of the direction of transcription of several of the reported genes. This contig will be instrumental for the detailed characterization of the tumor-associated chromosomal breakpoints and the identification of other 11q13-associated disease genes. (C) 2000 Academic Press.