A Whole Cell E. coli Display Platform for Artificial Metalloenzymes: Poly(phenylacetylene) Production with a Rhodium-Nitrobindin Metalloprotein
A Whole Cell E. coli Display Platform for Artificial Metalloenzymes: Poly(phenylacetylene) Production with a Rhodium-Nitrobindin Metalloprotein
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DOI：
10.1021/acscatal.7b04369
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发表时间：
2018-03-01
期刊：
影响因子：
12.9
通讯作者：
Schwaneberg, Ulrich
中科院分区：
文献类型：
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作者：
Grimm, Alexander R.;Sauer, Daniel F.;Schwaneberg, Ulrich
在许多情况下，全细胞催化是通过生物技术手段以成本效益高的方式生产化学品的先决条件。用于生物正交反应的合成金属催化剂可由于丰富的硫醇衍生物而在细胞内失活。在这里，细胞表面展示为基础的全细胞生物杂合催化剂系统（称为ArMt臭虫）被报道为一个普遍适用的平台，以统一具有成本效益的全细胞催化与生物杂合催化。采用失活的酯酶自转运蛋白在E. coli表面。对于ArMt bugs转化平台，苯乙炔的立体选择性聚合产生高转换数（TON）（39 X 10（6）细胞（-1））。
Whole cell catalysis is, in many cases, a prerequisite for the cost-effective production of chemicals by biotechnological means. Synthetic metal catalysts for bioorthogonal reactions can be inactivated within cells due to abundant thiol derivatives. Here, a cell surface display-based whole cell biohybrid catalyst system (termed ArMt bugs) is reported as a generally applicable platform to unify cost-effective whole cell catalysis with biohybrid catalysis. An inactivated esterase autotransporter is employed to display the nitrobindin protein scaffold with a Rh catalyst on the E. coli surface. Stereoselective polymerization of phenylacetylene yielded a high turnover number (TON) (39 X 10(6) cell(-1)) for the ArMt bugs conversion platform.