Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region.
Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region.
复制标题
枯草芽孢杆菌染色体复制起点区域的结构和功能。
DOI：
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复制
发表时间：
1985
影响因子：
14.9
通讯作者：
Hiroshi Yoshikawa
中科院分区：
文献类型：
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作者：
Shigeki Moriya;Naotake Ogasawara;Hiroshi Yoshikawa
在枯草芽孢杆菌染色体的oriC区域中对大约10，000个核苷酸进行测序。第一复制DNA链与SalI-EcoRI片段（核苷酸#1206-2954）在一个方向（从左到右）杂交，与EcoRI-Pst I片段（#2949-4233）在另一个方向杂交。鉴定出7个开放阅读框（ORF），并伴有SD序列。根据遗传学证据和氨基酸序列数据，ORF 638和ORF 821分别被鉴定为gyrB和gyrA基因。ORF 44、ORF 446、ORF 378和ORF 323分别与大肠杆菌的rpmH、dnaA、dnaN和recF同源。因此，从ORF 44到ORF 638的ORF的组织类似于E. coli染色体。在第一个复制DNA起始位点附近发现了oriC信号的两个非编码区特征。它们由重复序列组成，其共有序列TTAT（C/A）CACA与大肠杆菌oriC中的4个重复序列相同。杆菌
Approximately 10,000 nucleotides were sequenced in the oriC region of the Bacillus subtilis chromosome. The first replicating DNA strands are hybridized with a SalI-EcoRI fragment (nucleotide #1206-2954) in one direction (left to right) and an EcoRI-PstI fragment (#2949-4233) in the other. Seven open reading frames (ORF) accompanied with Shine-Dalgarno (SD) sequences were identified. ORF638 and ORF821 were identified as gyrB and gyrA genes respectively based on genetic evidences and amino acid sequence data. Comparison of amino acid sequences revealed that ORF44, ORF446, ORF378 and ORF323 are homologous with rpmH, dnaA, dnaN and recF of Escherichia coli, respectively. Thus, the organization of the ORFs from ORF44 to ORF638 resembles the organization of genes in the rpmH-gyrB region of the E. coli chromosome. Two non-coding regions characteristic for oriC signals were found near the site of initiation of the first replicating DNA. They are composed of repeating sequences whose consensus sequence TTAT(C/A)CACA is identical to that of 4 repeating sequences in the oriC of E. coli.