Rat renal, aortic and pulmonary endothelin-I receptors: effects of changes in sodium and water intake.
Rat renal, aortic and pulmonary endothelin-I receptors: effects of changes in sodium and water intake.
复制标题
大鼠肾、主动脉和肺内皮素-I 受体：钠和水摄入量变化的影响。
作者：
H. Michel;A. Bäcker;H. Meyer;I. Migas;H. Kramer
1. 在本研究中，我们首先研究了高 Na+ 摄入量的影响，其次研究了缺水对 32 只雌性 Sprague-Dawley 大鼠血浆内皮素-1 浓度和尿液内皮素-1 排泄以及肾小球和乳头膜以及主动脉平滑肌和肺组织内皮素受体的影响。 2. 5 周高 Na+ 摄入量 (n = 8) 后，尿 Na+ 排泄量为 10.5 +/- 1.3，而对照组为 1.6 +/- 0.2 mmol/24 小时。体重、血浆渗透压、血浆内皮素-1浓度（23+/-6与28+/-3fmol/ml）和尿内皮素-1排泄量（6.1+/-1.3与4.7+/-0.3pmol/24小时）保持不变。 3.盐负荷大鼠肾小球、乳头、主动脉平滑肌和肺组织内皮素1受体特征与对照组无差异。 4. 与对照组（n = 8）相比，禁水 48 小时后（n = 8）体重下降，而细胞压积、血浆和尿液渗透压增加（P < 0.05）。与对照组相比，血浆内皮素-1 浓度（40 +/- 6 与 21 +/- 2 fmol/ml）较高（P < 0.01），尿内皮素-1 排泄量（1.0 +/- 0.2 与 2.8 +/- 0.3 pmol/24 小时）较低（P < 0.01）。（摘要截短为 250 字）
1. In the present study we investigated, first, the effects of high Na+ intake and, second, the effects of water deprivation on plasma endothelin-1 concentration and urinary endothelin-1 excretion and on endothelin receptors in membranes of renal glomeruli and papillae and of aortic smooth muscle and lung tissue from 32 female Sprague-Dawley rats. 2. After 5 weeks of high Na+ intake (n = 8) urinary Na+ excretion was 10.5 +/- 1.3 compared with 1.6 +/- 0.2 mmol/24 h in controls. Body weight, plasma osmolarity, plasma endothelin-1 concentration (23 +/- 6 versus 28 +/- 3 fmol/ml) and urinary endothelin-1 excretion (6.1 +/- 1.3 versus 4.7 +/- 0.3 pmol/24 h) remained unchanged. 3. The characteristics of endothelin-1 receptors in glomeruli, papillae, aortic smooth muscle and lung tissue from salt-loaded rats were not different from those of controls. 4. After 48 h water deprivation (n = 8) body weight had decreased, whereas packed cell volume and plasma and urine osmolarities had increased compared with controls (n = 8) (P < 0.05). Plasma endothelin-1 concentration (40 +/- 6 versus 21 +/- 2 fmol/ml) was higher (P < 0.01) and urinary endothelin-1 excretion (1.0 +/- 0.2 versus 2.8 +/- 0.3 pmol/24 h) was lower than in controls (P < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)