Bioprospecting Sponge-Associated Microbes for Antimicrobial Compounds.
Bioprospecting Sponge-Associated Microbes for Antimicrobial Compounds.
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DOI：
10.3390/md14050087
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发表时间：
2016-05-02
期刊：
影响因子：
5.4
通讯作者：
Sipkema D
中科院分区：
文献类型：
--
作者：
Indraningrat AA;Smidt H;Sipkema D
海绵是最多产的海洋生物体，其生物活性化合物包括抗菌剂。然而，这些物质中的大多数可能不是由海绵本身产生的，而是由与其宿主相关的细菌或真菌产生的。这篇综述首次全面概述了已知由海绵相关微生物产生的抗菌化合物。我们讨论了目前的国家的最先进的分组海绵相关微生物产生的生物活性化合物分为四类：抗病毒，抗菌，抗真菌和抗原动物化合物。基于体外活性试验，已确定的源自海绵相关微生物的强效抗菌物质的靶标包括：人类免疫缺陷病毒1（HIV-1）（2-十一烷基-4-喹诺酮、山梨内酯A和查他鲁丁B）;甲型流感（H1N1）病毒（截短甘油M）;院内革兰氏阳性菌（硫肽YM-266183、YM-266184、mayamycin和kocurin）;大肠杆菌（sydonic acid）、沙眼衣原体（Chlamydia trachomatis）（并四苯糖苷SF 2446 A2）;疟原虫属（Plasmodium spp.）（曼扎明A和喹诺酮1）;杜氏利什曼原虫（曼扎明A和缬氨霉素）;布氏锥虫（缬氨霉素和星形孢菌素）;白色念珠菌和皮肤癣菌（saadamycin，5，7-二甲氧基-4-对甲氧苯基香豆素和YM-202204）。与海绵相关的35种细菌和12种真菌属被鉴定为产生抗菌剂，其中链霉菌属、假弧菌属、芽孢杆菌属、曲霉属和青霉属是抗菌化合物的主要生产者。进一步培养独立的方法，更全面地利用海绵相关细菌的抗菌化合物生产途径的遗传丰富性。
Sponges are the most prolific marine organisms with respect to their arsenal of bioactive compounds including antimicrobials. However, the majority of these substances are probably not produced by the sponge itself, but rather by bacteria or fungi that are associated with their host. This review for the first time provides a comprehensive overview of antimicrobial compounds that are known to be produced by sponge-associated microbes. We discuss the current state-of-the-art by grouping the bioactive compounds produced by sponge-associated microorganisms in four categories: antiviral, antibacterial, antifungal and antiprotozoal compounds. Based on in vitro activity tests, identified targets of potent antimicrobial substances derived from sponge-associated microbes include: human immunodeficiency virus 1 (HIV-1) (2-undecyl-4-quinolone, sorbicillactone A and chartarutine B); influenza A (H1N1) virus (truncateol M); nosocomial Gram positive bacteria (thiopeptide YM-266183, YM-266184, mayamycin and kocurin); Escherichia coli (sydonic acid), Chlamydia trachomatis (naphthacene glycoside SF2446A2); Plasmodium spp. (manzamine A and quinolone 1); Leishmania donovani (manzamine A and valinomycin); Trypanosoma brucei (valinomycin and staurosporine); Candida albicans and dermatophytic fungi (saadamycin, 5,7-dimethoxy-4-p-methoxylphenylcoumarin and YM-202204). Thirty-five bacterial and 12 fungal genera associated with sponges that produce antimicrobials were identified, with Streptomyces, Pseudovibrio, Bacillus, Aspergillus and Penicillium as the prominent producers of antimicrobial compounds. Furthemore culture-independent approaches to more comprehensively exploit the genetic richness of antimicrobial compound-producing pathways from sponge-associated bacteria are addressed.