MICRONUCLEUS INDUCTION IN PERIPHERAL-BLOOD LYMPHOCYTES OF PATIENTS UNDER RADIOTHERAPY TREATMENT FOR CERVICAL-CANCER OR HODGKINS-DISEASE
MICRONUCLEUS INDUCTION IN PERIPHERAL-BLOOD LYMPHOCYTES OF PATIENTS UNDER RADIOTHERAPY TREATMENT FOR CERVICAL-CANCER OR HODGKINS-DISEASE
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DOI：
10.1080/09553009514550641
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发表时间：
1995-05-01
影响因子：
2.6
通讯作者：
DERIDDER, L
中科院分区：
文献类型：
--
作者：
THIERENS, H;VRAL, A;DERIDDER, L
在接受宫颈癌或霍奇金病分次局部放射治疗的患者的外周血淋巴细胞中存在的遗传损伤在治疗过程中通过胞质分裂阻断微核测定进行了跟踪。对于每个患者，还确定了血液样本预处理后体外辐射后微核数量的剂量-反应关系。比较个体体内和体外数据，发现放射治疗期间等效全身剂量后的微核产量与体外剂量反应有很大不同。与体外辐射后的线性二次剂量依赖性相反，放射治疗期间微核产量的初始增加在剂量升高时趋于平稳。观察到的差异不能仅归因于间期死亡和淋巴细胞池部分照射的影响。在放射治疗第一部分的汇总数据中观察到的微核产量与高达 2 Gy 的等效全身剂量之间的相关性，证明了胞质分裂阻断微核测定作为涉及部分身体照射事故后的生物剂量计的适用性。
The genetic damage present in peripheral blood lymphocytes of patients treated with fractionated partial-body radiation therapy for cervical cancer or Hodgkin's disease was followed during treatment by means of the cytokinesis-block micronucleus assay. For each patient a dose-response relationship with respect to the number of micronuclei after in vitro irradiation of blood samples pretreatment was also determined. Comparing the individual in vivo-in vitro data, the micronucleus yields after the equivalent whole-body dose during radiotherapy were found to differ substantially from the in vitro dose-response. Contrary to the linear-quadratic dose dependence after in vitro irradiation the initial increase in the micronucleus yield during radiotherapy levelled off at elevated doses. The observed differences cannot be attributed only to the effects of interphase death and the partial irradiation of the lymphocyte pool. The correlation between the micronucleus yield and the equivalent whole-body dose for values up to 2 Gy, observed for the pooled data of the first part of the radiotherapy treatment, demonstrates the suitability of the cytokinesis-block micronucleus assay as a biological dosemeter after accidents involving partial-body irradiation.