Superresolution microscopy reveals actomyosin dynamics in medioapical arrays.
Superresolution microscopy reveals actomyosin dynamics in medioapical arrays.
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DOI：
10.1091/mbc.e21-11-0537
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发表时间：
2022-09-15
影响因子：
3.3
通讯作者：
Kiehart, Daniel P.
中科院分区：
文献类型：
--
作者：
Moore, Regan P.;Fogerson, Stephanie M.;Tulu, U. Serdar;Yu, Jason W.;Cox, Amanda H.;Sican, Melissa A.;Li, Dong;Legant, Wesley R.;Weigel, Aubrey, V;Crawford, Janice M.;Betzig, Eric;Kiehart, Daniel P.
在整个系统发育过程中，靠近上皮细胞顶端表面的肌动蛋白细丝(F-肌动蛋白)阵列(中尖阵列)有助于顶端收缩和形态发生。在这里，超分辨方法(掠入射结构照明，GI-SIM，和晶格光片，LLSM)显微镜解析单独的，荧光标记的F-肌动蛋白和双极肌球蛋白细丝，这些细丝在果蝇的背部闭合过程中驱动羊膜绒毛细胞形状的变化。在扩张的细胞中，F-肌动蛋白和肌球蛋白在质膜上形成疏松的顶端穹顶网状结构。随着细胞收缩，阵列浓缩，将穹顶拉入连接带的平面。随着凝结的继续，单个细丝不再均匀地显现。随着细胞的扩张，肌动球蛋白的阵列再次被分解--可能会发生一些F-肌动蛋白的转换，但现有细丝的很大一部分会重新排列。在形态各向同性的细胞中，肌动蛋白细丝是随机定向的，在收缩过程中被拉在一起，但基本上保持随机定向。在各向异性细胞中，大部分平行的肌动蛋白细丝相互拉近。我们的图像提供了无与伦比的F-肌动蛋白在胚胎组织中的分辨率，表明中尖阵列与质膜紧密相对，并与层状F-肌动蛋白网状结构连续。因此，中根尖排列构成了改良的细胞皮质。结合其他标记阵列组件，活标本的超分辨率成像将提供对皮质结构和功能的新理解。
Arrays of actin filaments (F-actin) near the apical surface of epithelial cells (medioapical arrays) contribute to apical constriction and morphogenesis throughout phylogeny. Here, superresolution approaches (grazing incidence structured illumination, GI-SIM, and lattice light sheet, LLSM) microscopy resolve individual, fluorescently labeled F-actin and bipolar myosin filaments that drive amnioserosa cell shape changes during dorsal closure in Drosophila. In expanded cells, F-actin and myosin form loose, apically domed meshworks at the plasma membrane. The arrays condense as cells contract, drawing the domes into the plane of the junctional belts. As condensation continues, individual filaments are no longer uniformly apparent. As cells expand, arrays of actomyosin are again resolved—some F-actin turnover likely occurs, but a large fraction of existing filaments rearrange. In morphologically isotropic cells, actin filaments are randomly oriented and during contraction are drawn together but remain essentially randomly oriented. In anisotropic cells, largely parallel actin filaments are drawn closer to one another. Our images offer unparalleled resolution of F-actin in embryonic tissue, show that medioapical arrays are tightly apposed to the plasma membrane and are continuous with meshworks of lamellar F-actin. Medioapical arrays thereby constitute modified cell cortex. In concert with other tagged array components, superresolution imaging of live specimens will offer new understanding of cortical architecture and function.
DOI：
10.1073/pnas.2109506119
发表时间：
2022-01-18
影响因子：
11.1
作者：
Wioland H;Jégou A;Romet-Lemonne G
通讯作者：
Romet-Lemonne G