An enzyme-powered, three-dimensional lame DNA walker
An enzyme-powered, three-dimensional lame DNA walker
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酶驱动的三维跛脚 DNA 行走者
DOI：
10.1016/j.bios.2021.112981
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发表时间：
2021
影响因子：
12.6
通讯作者：
Xie Guoming
中科院分区：
文献类型：
--
作者：
Fang Jie;Yuan Changjing;Li Junjie;Li Junlong;Yang Tingyan;Guo Yongcan;Wang Ding;Xue Jianjiang;Fu WeiLing;Xie Guoming
由三维（3-D）DNA步行者构建的分子机器已经成为诸如新型生物传感器、货物递送平台和细胞内成像的应用中的热门话题。在此，我们首先提出了一个跛脚的DNA步行者，可以随机和自主移动的微球为基础的三维轨道。随机跛行步行者具有主要负责持续运动的长腿和快速切割底物的短腿。它的运动是由杂交DNA轨道的切口核酸内切酶切割推动的。动力学和持久性研究表明，跛脚DNA步行者能够在30 min内达到反应平衡，需要至少14个核苷酸的cleat结构域，并且能够以6.467 × 10− 11 M s−1的平均速率在三维轨道上持续移动。我们还证明，跛脚步行者可用于检测目标DNA的检测范围为10 pM-5 nM的高特异性的立足点交换机制。这项工作将进一步扩展3-D DNA步行器的性能，并大大有助于提高对DNA步行系统的理解。
Molecular machines constructed by three-dimensional (3-D) DNA walker have emerged as a hot topic in applications such as novel biosensors, cargo delivery platforms and intracellular imaging. Herein, we first propose a lame DNA walker that can randomly and autonomously move on microsphere-based 3-D track. The stochastic lame walker has a long leg mainly responsible for persistent movement and a short leg cutting substrates rapidly. Its motion is propelled by a nicking endonuclease cleavage of hybridized DNA tracks. Kinetic and persistent study show that the lame DNA walker enables reaction equilibrium at 30 min, need a cleat domain of at least 14 nucleotides and can persistently move on 3-D tracks with an average rate of 6.467 × 10−11M s−1. We also demonstrate that the lame walker can be used to detect target DNA in the detection range of 10 pM–5 nM with high specificity by toehold exchange mechanism. This work will further expand the performance of 3-D DNA walkers and substantially contributes to the improved understanding of DNA walking systems.