Puerarin enhances superoxide dismutase activity and inhibits RAGE and VEGF expression in retinas of STZ-induced early diabetic rats
Puerarin enhances superoxide dismutase activity and inhibits RAGE and VEGF expression in retinas of STZ-induced early diabetic rats
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DOI：
10.1016/s1995-7645(12)60166-7
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发表时间：
2012-11-01
影响因子：
3.1
通讯作者：
Xie, Zheng-Gao
中科院分区：
文献类型：
--
作者：
Chen, Fang;Zhang, Hong-Quan;Xie, Zheng-Gao
目的：观察葛根素对链脲佐菌素（STZ）诱导的早期糖尿病大鼠视网膜超氧化物歧化酶（SOD）活性、糖基化终产物（AGE）受体（AGE）和血管内皮生长因子（VEGF）表达的影响。方法：腹腔注射链脲佐菌素（STZ）诱导糖尿病大鼠模型。将大鼠随机分为正常对照组、糖尿病组和糖尿病+葛根素组。葛根素灌胃（500 mg/kg/d，连续4周）后，测定血清和视网膜中SOD和MDA的含量。采用实时荧光定量PCR（RT-PCR）和蛋白质印迹法（Western blot）分别检测视网膜中VEGF和VEGF的mRNA和蛋白质表达水平。结果如下：DM组血清和视网膜中SOD活性明显低于其他两组（P
Objective: To investigate the effects of puerarin on the activity of superoxide dismutase (SOD), and expressions of advanced glycation end-product (AGE) receptor (RAGE) and vascular endothelial growth factor (VEGF) in retinas of streptozotocin (STZ)-induced early diabetic rats. Methods: Diabetic rat models were established by inducing diabetes via intra-peritoneal injection of STZ. Rats were randomly divided into normal (control), diabetic (DM), and DM+ puerarin groups. After intra-gastric administration of puerarin (500 mg/kg/day for 4 weeks), levels of SOD and malondialdehyde (MDA) were determined in serum and retina. mRNA and protein expression levels of RAGE and VEGF in retinas were determined by real-time polymerase chain reaction (RT-PCR) (mRNA) and Western blot analysis (protein levels). Results: There was significantly lower SOD activity and significantly higher MDA in serum and retinas of the DM group compared with the two other groups (P