Characterization of alcohol oxidase from Aspergillus ochraceus AIU 031
Characterization of alcohol oxidase from Aspergillus ochraceus AIU 031
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DOI：
10.2323/jgam.53.177
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发表时间：
2007-06-01
影响因子：
1.2
通讯作者：
Shimizu, Sakayu
中科院分区：
文献类型：
--
作者：
Isobe, Kimiyasu;Kato, Ayako;Shimizu, Sakayu
从赭曲霉AIU 031中分离到一种醇氧化酶（AOD），并对其特性进行了研究。该酶氧化短链伯醇和乙二醇，与甲基营养型酵母中的AOD属于同一组。但是，它在以下属性上有所不同。乙醇的K_i值较大，乙二醇的K_i值较小。乙醇氧化在pH 5-7和50-550 ℃下是最佳的。该酶的分子量为262 kDa，由4个相同的68 kDa的亚基组成，这是远远小于那些甲基营养型酵母。
An alcohol oxidase (AOD) was found from Aspergillus ochraceus AIU 031, and its characteristics were revealed. This enzyme oxidized short-chain primary alcohols and ethylene glycol, and belonged to the same group as AOD from methylotrophic yeast. However, it differed in the following properties. The K, value for ethanol was,larger and that for ethylene glycol was smaller than those of AODs derived from methylotrophic yeasts. The ethanol oxidation was optimal at pH 5-7 and 50-550 degrees C. The molecular mass of this enzyme was 262 kDa and consisted of four identical subunits of 68 kDa, which were much smaller than those of methylotrophic yeasts.