Arabidopsis TOR signaling is essential for sugar-regulated callus formation
Arabidopsis TOR signaling is essential for sugar-regulated callus formation
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DOI：
10.1111/jipb.12560
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发表时间：
2017-10-01
影响因子：
11.4
通讯作者：
Seo, Pil Joon
中科院分区：
文献类型：
--
作者：
Lee, Kyounghee;Seo, Pil Joon
脱分化是一个显着的过程，从分化的体细胞产生多能干细胞，以确保发育可塑性。植物已经进化出细胞脱分化的能力，与生长素和细胞分裂素依赖的愈伤组织形成相关的信号级联已被广泛研究。然而，糖依赖愈伤组织形成的分子机制仍不清楚。在这里，我们证明了糖依赖的愈伤组织的形成主要由拟南芥中的TOR-E2Fa模块调控。糖激活的TOR激酶在愈伤组织形成过程中磷酸化和稳定E2Fa蛋白，以转录方式激活S期基因。同时，E2Fa受到ARF-LBD转录级联的转录调控。糖和生长素对E2Fa的多层调控可能会形成拟南芥平衡的细胞去分化能力。
Dedifferentiation is a remarkable process that produces pluripotent stem cells from differentiated somatic cells to ensure developmental plasticity. Plants have evolved the ability of cellular dedifferentiation, and signaling cascades related to auxin and cytokinin-dependent callus formation have been extensively investigated. However, the molecular mechanism underlying sugar-dependent callus formation remains unknown. Here, we show that sugar-dependent callus formation is mainly regulated by the TOR-E2Fa module in Arabidopsis. Sugar-activated TOR kinase phosphorylates and stabilizes E2Fa proteins to transcriptionally activate S-phase genes during callus formation. In parallel, E2Fa is transcriptionally regulated by the ARF-LBD transcription cascade. Multi-layered regulation of E2Fa by sugar and auxin is likely to shape balanced cellular dedifferentiation capability in Arabidopsis.