Shifts in diversity and community structure of endophytic bacteria and archaea across root, stem and leaf tissues in the common reed, Phragmites australis, along a salinity gradient in a marine tidal wetland of northern China
Shifts in diversity and community structure of endophytic bacteria and archaea across root, stem and leaf tissues in the common reed, Phragmites australis, along a salinity gradient in a marine tidal wetland of northern China
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DOI：
10.1007/s10482-013-9984-3
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发表时间：
2013-11-01
影响因子：
2.6
通讯作者：
Gong, Jun
中科院分区：
文献类型：
--
作者：
Ma, Bin;Lv, Xiaofei;Gong, Jun
盐胁迫对植物内生原核生物群落的影响在很大程度上是未知的，细菌和古细菌内生菌在植物物种的不同组织中的分布模式很少比较。我们调查了内生细菌和古细菌群落的根，茎和叶的共同芦苇，芦苇，收集从三个潮汐带沿着一个盐度梯度，利用末端限制性片段（T-RF）长度多态性分析的16 S rRNA基因。结果表明，根中的细菌多样性显著高于叶中，而植物组织和潮间带的古细菌多样性相似。网络分析显示，T-RF主要按组织分组，主要组通常由几个共同的T-RF连接。根中的T-RFs主要存在于潮上带的植物中，而茎和叶中的T-RFs主要存在于中、高潮带的植物中。非度量多维标度排序和相似性分析表明，各组织间细菌群落差异显著（P < 0.05），而各潮间带间细菌群落相似（P = 0.49）。不同潮间带的古菌群落差异显著（P < 0.05），但不同组织间的古菌群落相似（P = 0.89）。这项研究表明：（2）内生细菌和内生古菌在植物组织中沿着不同盐度梯度的分布格局表明，这两个类群在滨海水生植物中起着不同的作用。
The effects of salt stress on endophytic prokaryotic communities in plants are largely unknown, and the distribution patterns of bacterial and archaeal endophytes in different tissues of a plant species are rarely compared. We investigated the endophytic bacterial and archaeal communities in roots, stems and leaves of the common reed, Phragmites australis, collected from three tidal zones along a salinity gradient, using terminal restriction fragment (T-RF) length polymorphism analysis of the 16S rRNA genes. The results showed that the bacterial diversity in the roots was significantly higher than that in the leaves, whereas similar archaeal diversity was revealed for either plant tissues or tidal zones. Network analysis revealed that T-RFs were grouped largely by tissue, and the major groups were generally linked by a few common T-RFs. Unique T-RFs in roots were mainly present in plants growing in the supratidal zone, but unique T-RFs in stems and leaves were mainly present in those from the middle and high tidal zones. Non-metric multidimensional scaling ordination and analysis of similarity revealed that bacterial communities were significantly different among tissues (P < 0.05), but similar among tidal zones (P = 0.49). However, the archaeal communities differed among tidal zones (P < 0.05), but were similar among tissues (P = 0.89). This study indicates that: (1) the endophytic archaeal communities are influenced more significantly than the endophytic bacterial communities by soil salinity, and (2) the differential distribution patterns of bacterial and archaeal endophytes in plant tissues along a salinity gradient imply that these two groups play different roles in coastal hydrophytes.