Analysis of BCR/ABL abnormalities in mRNA from 20-year-old paraffin-embedded tissue for BCR/ABL rearrangement by polymerase chain reaction.
Analysis of BCR/ABL abnormalities in mRNA from 20-year-old paraffin-embedded tissue for BCR/ABL rearrangement by polymerase chain reaction.
复制标题
通过聚合酶链式反应分析 20 年前石蜡包埋组织中 BCR/ABL mRNA 异常的 BCR/ABL 重排。
DOI：
10.1159/000204364
复制
发表时间：
1993
影响因子：
2.4
通讯作者：
R. Gale
中科院分区：
文献类型：
--
作者：
I. Aurer;T. Juhbashi;I. Sekine;M. Tomonaga;R. Gale
We studied whether it is possible to obtain sufficient mRNA from old paraffin-embedded samples of spleen and bone marrow for reverse transcription and polymerase chain reaction analysis. Spleen tissue from 6 subjects with chronic myelogenous leukemia was studied for rearrangement of BCR and ABL genes. Analysis was successful in 4 cases. These data indicate the feasibility of retrospective analysis of tissue samples of special interest by molecular techniques.
