Collagen-carbon nanotube composite materials as scaffolds in tissue engineering
Collagen-carbon nanotube composite materials as scaffolds in tissue engineering
复制标题
DOI：
10.1002/jbm.a.30386
复制
发表时间：
2005-09-01
影响因子：
4.9
通讯作者：
Stegemann, JP
中科院分区：
文献类型：
--
作者：
MacDonald, RA;Laurenzi, BF;Stegemann, JP
碳纳米管(CNT)由于具有很高的长径比以及优异的机械和电学性能，在纤维增强复合材料中的应用具有很大的吸引力。将溶解的I型胶原与浓度分别为0、0.2、0.4、0.8和2.0重量百分比的羧化单壁碳纳米管(SWNT)溶液混合，制备了含有嵌入CNT的胶原基质的复合材料。在胶原凝胶形成时，将活的平滑肌细胞掺入，形成细胞种植的胶原-碳纳米管复合基质。含有2.0wt%碳纳米管的构建物显示出延迟的凝胶压实，相对于以与纯胶原对照相同的速度压实的较低浓度。在第3天和第7天，所有构建物的细胞存活率都保持在85%以上，而含CNT构建物的细胞数量在第3天低于对照构建物，但到第7天在统计学上没有变化。扫描电子显微镜显示CNT与胶原基质之间存在物理相互作用。拉曼光谱证实了预期直径(0.85-1.30 nm)的碳纳米管的存在，但没有表明胶原和碳纳米管成分之间存在强烈的分子相互作用。这种胶原-碳纳米管复合材料基质可用作组织工程中的支架，或用作生物传感器或其他医疗设备的组件。(C)2005年威利期刊公司。
Carbon nanotubes (CNT) are attractive for use in fiber-reinforced composite materials due to their very high aspect ratio, combined with outstanding mechanical and electrical properties. Composite materials comprising a collagen matrix with embedded CNT were prepared by mixing solubilized Type I collagen with solutions of carboxylated single-walled carbon nanotubes (SWNT) at concentrations of 0, 0.2, 0.4, 0.8, and 2.0 weight percent. Living smooth muscle cells were incorporated at the time of collagen gelation to produce cell-seeded collagen-CNT composite matrices. Constructs containing 2.0 wt% CNT exhibited delayed gel compaction, relative to lower concentrations that compacted at the same rate as pure collagen controls. Cell viability in all constructs was consistently above 85% at both Day 3 and Day 7, whereas cell number in CNT-containing constructs was lower than in control constructs at Day 3, though statistically unchanged by Day 7. Scanning electron microscopy showed physical interactions between CNT and collagen matrix. Raman spectroscopy confirmed the presence of CNT at the expected diameter (0.85-1.30 nm), but did not indicate strong molecular interactions between the collagen and CNT components. Such collagen-CNT composite matrices may have utility as scaffolds in tissue engineering, or as components of biosensors or other medical devices. (c) 2005 Wiley Periodicals, Inc.