Negative regulation by transcription factor VvWRKY13 in drought stress of Vitis vinifera L
Negative regulation by transcription factor VvWRKY13 in drought stress of Vitis vinifera L
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转录因子VvWRKY13对葡萄干旱胁迫的负调控
DOI：
10.1016/j.plaphy.2020.01.008
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发表时间：
2020-03-01
影响因子：
6.5
通讯作者：
Liu, Xin
中科院分区：
文献类型：
--
作者：
Hou, Lixia;Fan, Xinxin;Liu, Xin
干旱是全球范围内限制作物生长发育的主要环境因素。WRKY转录因子是植物中特有的一种转录因子，在植物对非生物胁迫的应答中发挥重要作用。此前，我们已经从抗病葡萄品种中克隆了VvWRKY13基因，发现其表达明显受干旱诱导。本文进一步探讨了VvWRKY13在干旱胁迫下的响应机制。干旱处理后，敏感品种中VvWRKY13的表达量显著高于抗性品种。此外，对转VvWRKY13基因拟南芥进行了干旱处理，并对干旱相关指标进行了检测。结果表明，与野生型相比，转VvWRKY13基因拟南芥表现出对干旱胁迫更敏感的表型。转基因拟南芥叶片失水速率显著高于野生型。干旱胁迫下，转基因拟南芥叶片中脯氨酸、可溶性糖含量及相关基因的表达量下降。内源性过氧化氢和氧自由基水平升高，过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性降低。此外，转基因拟南芥中胁迫应答基因的表达量显著降低。综上所述，我们的研究结果表明，VvWRKY13通过调节细胞内渗透物质（脯氨酸，可溶性糖）的代谢，ROS的水平，和胁迫相关基因的表达负调节植物耐旱性。
Drought is a major environmental factor limiting crop growth and development worldwide. WRKY transcription factor, a unique transcription factor in plants, has been shown to play important roles in plant response to abiotic stress. Previously, we have cloned the VvWRKY13 gene from resistant grape varieties and found that its expression was obviously induced by drought. Here we further explored the mechanism of VvWRKY13 in response to drought stress. After drought treatment, the expression of VvWRKY13 in the sensitive grape varieties was significantly higher than resistant grape varieties. Moreover, phenotypic changes of VvWRKY13 transgenic Arabidopsis were observed and drought-related indexes were detected under drought treatment. The results showed that VvWRKY13 transgenic Arabidopsis exhibited more sensitive phenotype to drought stress compared with wild type. The water loss rate of leaves in the transgenic Arabidopsis was significantly higher than wild type. The content of proline, soluble sugar and the expression of related genes decreased in transgenic Arabidopsis leaves under drought stress. The level of endogenous hydrogen peroxide and oxygen free radicals was increased, while the activity of catalase (CAT) and superoxide dismutase enzyme (SOD) were decreased. In addition, the expression of stress response gene was significantly decreased in transgenic Arabidopsis. Taken together, our results suggest that VvWRKY13 negatively modulates plant drought tolerance through regulating the metabolism of intracellular osmotic substances (proline, soluble sugar), the level of ROS, and the expression of stress-related genes.