TRANSFECTION BY EXOGENOUS AND ENDOGENOUS MURINE RETROVIRUS DNAS
TRANSFECTION BY EXOGENOUS AND ENDOGENOUS MURINE RETROVIRUS DNAS
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DOI：
10.1016/0092-8674(79)90011-4
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发表时间：
1979-01-01
期刊：
影响因子：
64.5
通讯作者：
COOPER, GM
中科院分区：
文献类型：
--
作者：
COPELAND, NG;COOPER, GM
用DNA转染法研究未感染小鼠细胞所遗传的内源性逆转录病毒基因组。应用外源性感染亲生性Moloney、AKR或BALB或异嗜性AKR、BALB或NZB类小鼠白血病病毒的细胞供体DNA，建立了亲生性和异嗜性小鼠白血病病毒(MuLV)感染性DNA的定性检测方法。外源感染的细胞DNA在NIH/3T3细胞上的转染率约为0.5个感染单位(IU)/µg DNA。外源感染生态型MuLV的细胞的DNA对水貂CCL64细胞的特异性感染力约为0.2IU/µg DNA。相反，未感染的NIH/3T3、BALB/3T3和AKR-2B小鼠细胞的DNA在检测感染性内源性亲生性MuLV DNA(<；0.001 IU/uMGDNA)时是非感染性的。同样，未感染NIH/3T3、BALB/3T3、AKR-2B和NZB-Q小鼠细胞的异质性小鼠病毒DNA的感染力比异质性小鼠病毒感染细胞的DNA感染力低100多倍。用Kirsten小鼠肉瘤病毒(MSV)或SV-40转化的BALB/3T3细胞的DNA对异嗜性和生理性小鼠白血病病毒均无感染性，但这些细胞含有感染性转化DNA或MSV或SV-40。因此，未感染的小鼠细胞的内源性MuLV基因组似乎与感染MuLV的细胞的MuLV前病毒不同。这些结果表明，内源MuLV基因组与细胞DNA序列相连，导致内源MuLV基因组转录效率低下，并降低了内源MuLV DNA的感染力。
DNA transfection was used to study the endogenous retrovirus genomes inherited by uninfected mouse cells. Qualitative assays for infectious DNA of ecotropic and xenotropic murine leukemia viruses (MuLV) were developed using donor DNA of cells that were exogenously infected with ecotropic Moloney, AKR or BALB MuLV, or with xenotropic AKR, BALB or NZB MuLV. The DNA of cells exogenously infected with ecotropic MuLV had specific infectivities of approximately 0.5 infectious units (IU)/.mu.g DNA in transfection assays on NIH/3T3 cells. The DNA of cells exogenously infected with ecotropic MuLV had specific infectivities of approximately 0.2 IU/.mu.g DNA in transfection assays on mink CCL64 cells. In contrast, the DNA of uninfected NIH/3T3, BALB/3T3 and AKR-2B mouse cells were noninfectious when assayed for infectious endogenous ecotropic MuLV DNA (< 0.001 IU/Umg DNA). Similarly, the infectivities of xenotropic MuLV DNAs of uninfected NIH/3T3, BALB/3T3, AKR-2B and NZB-Q mouse cells were more than 100-fold lower than the infectivities of DNA of xenotropic MuLV-infected cells. The DNA of BALB/3T3 cells transformed by Kirsten murine sarcoma virus (MSV) or by SV-40 were noninfectious for either ecotropic or xenotropic MuLV, although these cells contained infectious transforming DNA or MSV or SV-40. The endogenous MuLV genomes of uninfected mouse cells thus appeared to differ from the MuLV proviruses of MuLV-infected cells. These results indicate that endogenous MuLV genomes are linked to cellular DNA sequences which result in both inefficient transcription of endogenous MuLV genomes and the reduced infectivity of endogenous MuLV DNA in transfection assays.