Hybrid genome assembly and gene repertoire of the root endophyte Clitopilus hobsonii QYL-10 (Entolomataceae, Agaricales, Basidiomycetes).
Hybrid genome assembly and gene repertoire of the root endophyte Clitopilus hobsonii QYL-10 (Entolomataceae, Agaricales, Basidiomycetes).
复制标题
DOI：
10.1094/mpmi-11-20-0328-a
复制
发表时间：
2021-02
期刊：
影响因子：
--
通讯作者：
Long Peng;Xiaoliang Shan;Yuchen Wang;F. Martin;R. Vilgalys;Zhilin Yuan
中科院分区：
文献类型：
--
作者：
Long Peng;Xiaoliang Shan;Yuchen Wang;F. Martin;R. Vilgalys;Zhilin Yuan
黑森阴蒂菌（Clitopilus hobsonii）是一种常见的土壤腐殖酸菌。也有证据表明，木臭草可以作为一个根内生菌有利于树木的生长。本文报道了从黑栎（Quercus lyrata）外生菌根根尖分离得到的C. hobsonii QYL-10的基因组组装。基因组大小为36.93 Mb，包含13个contigs (N50=3.3 Mb)， gc含量为49.2%。其中，10个片段接近完整染色体的长度，3个片段只有一端有端粒。BUSCO分析报告使用担子菌ta_odb10的完整性评分为98.4%。结合ab-initio， RNA-seq数据和基于同源性的预测，我们确定了12,710个蛋白质编码基因。共鉴定出1.43 Mb的转座因子（te）（3.88%）， 36个次生代谢物生物合成基因簇和361个编码推定CAZymes的基因。这一基因组资源将有助于开展功能研究，目的是表征黑梭菌与其寄主树之间的共生相互作用，但也将为进一步开展狐卷菌科比较基因组学研究提供有价值的基础。
Clitopilus hobsonii (Entolomataceae, Agaricales, Basidiomycetes) is a common soil saprotroph. There is also evidence that C. hobsonii can act as a root endophyte benefiting tree growth. Here, we report the genome assembly of C. hobsonii QYL-10 isolated from ectomycorrhizal root tips of Quercus lyrata. The genome size is 36.93 Mb, consisting of 13 contigs (N50=3.3 Mb) with 49.2% GC-content. Of them, 10 contigs approached the length of intact chromosomes, and 3 had telomeres at one end only. BUSCO analysis reported a completeness score of 98.4% using the Basidiomycota_odb10. Combining ab-initio, RNA-seq data, and homology-based predictions, we identified 12,710 protein-coding genes. Approximately, 1.43 Mb of Transposable elements (TEs) (3.88% of the assembly), 36 secondary metabolite biosynthetic gene clusters and 361 genes encoding putative CAZymes were identified. This genomic resource will allow functional studies aimed to characterize the symbiotic interactions between C. hobsonii and its host trees, but will also provide a valuable foundation for further research on comparative genomics of the Entolomataceae.