Comprehensive characterization of the effect of tissue storage conditions on tissue-adhesive interaction
Comprehensive characterization of the effect of tissue storage conditions on tissue-adhesive interaction
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DOI：
10.1163/156856208786140337
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发表时间：
2008-01-01
影响因子：
3.6
通讯作者：
Maziarz, Peter E.
中科院分区：
文献类型：
--
作者：
Samuel, Newton T.;Vailhe, Elizabeth;Maziarz, Peter E.
在组织粘合剂的评价中，新鲜采集的组织与临床应用最相关;然而，很难在手术后立即获得和测试实验样品。本研究的目的是阐明储存条件对保存的组织样品模仿新鲜收获组织的能力的影响。猪小肠被用作实验的模型组织。处理条件为新鲜收获，在5 ℃磷酸盐缓冲盐水中老化24小时，福尔马林固定组织。通过T-剥离测试表征氰基丙烯酸酯和新合成的组织粘合剂在三种基材上的粘合性能。无论使用何种粘合剂，新鲜组织样本的粘合强度最弱。1天老化组织产生的粘合强度约为两倍，福尔马林固定的肠基质具有中等粘合强度。采用衰减全反射红外（ATR-IR）光谱、捕获气泡接触角、拉曼光谱、大体积组织含水量测量和组织学进行综合组织表征。来自不同表征技术的结果表明，组织样品的储存导致界面和化学变化，限制了它们模仿新鲜采集组织的能力。特别地，结果显示了通过ATR-IR检测到的水在介导对组织样品的粘附中的重要性。
In the evaluation of tissue adhesives, freshly harvested tissues are the most relevant to clinical application; however, it is difficult to obtain and test experimental samples immediately following surgery. The aim of the present investigation is to elucidate the effect of storage conditions on the ability of preserved tissue samples to mimic freshly harvested tissue. Porcine small intestine was used as a model tissue for the experiments. The treatment conditions were freshly harvested, 24-h aged in phosphate-buffered saline at 5 degrees C and formalin-fixed tissue. Adhesive performance of cyanoacrylate and a newly synthesized tissue adhesive on the three substrates was characterized by T-peel testing. Regardless of the adhesive used, the fresh tissue samples had the weakest bond strength. The 1-day aged tissue produced a bond that was approximately twice as strong, and the formalin-fixed intestine substrate had intermediate bond strength. Comprehensive tissue characterization using attenuated total reflection infrared (ATR-IR) spectroscopy, captive-bubble contact angle, Raman spectroscopy, bulk tissue water content measurement and histology were employed. The results from the different characterization techniques show that storage of tissue samples causes interfacial and chemical changes, limiting their ability to mimic freshly harvested tissue. In particular, the results show the importance of water detected by ATR-IR in mediating adhesion to the tissue samples.