ISOLATION OF BORDETELLA PERTUSSIS AND BORDETELLA PARAPERTUSSIS OF CLINICAL SPECIMENS FROM DIFFERENT PROVINCES OF IRAN
ISOLATION OF BORDETELLA PERTUSSIS AND BORDETELLA PARAPERTUSSIS OF CLINICAL SPECIMENS FROM DIFFERENT PROVINCES OF IRAN
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伊朗各省临床标本中百日咳博德特氏菌和副百日咳博德特氏菌的分离
DOI：
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Z. Mohsen
中科院分区：
文献类型：
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作者：
S. Fereshteh;Nakhost Lotfi Masoumeh;Parzadeh Masoumeh;Nikbin Vajiheh Sadat;Shooraj Fahimeh;Z. Mohsen
背景和目的：百日咳博德泰拉是一种革兰氏阴性和专性需氧细菌，是百日咳的原因，是唯一的人类病原体。在过去十年中，观察到百日咳的发病率上升。尽管百日咳作为一种传染病很重要，但关于其在伊朗的发病率，没有足够的资料。本研究对2009-2010年在伊朗不同省份采集的可疑百日咳标本进行了研究。材料与方法：收集伊朗不同省份2周以上咳嗽患者鼻咽分泌物或鼻腔标本1084份，送往伊朗巴斯德研究所百日咳参比实验室微生物组。采用培养法进行分离。所有样品均接种于含头孢氨苄抗生素（40µg/ml）和不含头孢氨苄抗生素的百日咳炭琼脂和博德-根沟板上。然后对疑似菌落进行革兰氏染色，如果菌落被鉴定为百日咳、副百日咳，则进行补充试验。结果：1084份接种标本中12份阳性（1.1%），其中百日咳白咳11份，副百日咳1份。培养阳性3例为2月龄以下患者，6例为2月龄至2岁个体，2 ~ 10岁2例，10岁以上1例。培养阳性标本接种9例，抗生素使用7例。结论：本研究结果表明，在伊朗分离的大多数菌株与2个月至2岁的儿童有关。针对伊朗地区百日咳的临床及临床旁问题，以及培养法诊断Bordotella的敏感性较低，应采用Real-Time PCR等辅助方法。
Background and purpose: Bordetella pertussis is a gram negative and obligate aerobic bacteria that is cause of whooping cough and is exclusively a human pathogen. In the last decade increasing rate of pertussis was observed. Despite the importance of pertussis as a contagious disease enough information does not exist regarding its incidence rate in Iran. In this research pertussis suspicious specimens were collected from different provinces of Iran in 2009-2010 and then were studied. Materials and methods: A total of 1084 sample from nasopharyngeal secretion or nasal of patients with coughing more than two weeks were collected from different provinces of Iran and were sent to microbiology unit of pertussis reference laboratory in Pasteur institute of Iran. The isolation was done through culturing method. All samples were inoculated through swab on pertussis charcoal agar and Bordet-Gengou plates containing (40 µg/ml) cephalexin antibiotic and once without it. Then the suspected colonies were gram stained and complementary tests was done if colonies were identified as B. pertussis, B. parapertussis. Results: From the total of 1084 samples 12 inoculated samples were positive (1.1%) of which 11 samples were B. pertussis and one sample was B. parapertussis. Among positive cultures, three cases were the patients who were below 2 months, six cases related to individuals from two months to two years of age, two cases related to those aged between two and 10 years old and one patient was over 10 years of age. Nine cases of positive culture specimens were vaccinated and seven cases were the patients who used antibiotics. Conclusion: Results of this study indicated that most isolated strains in Iran were related to those aged between two months to two years old. According to clinical and paraclinical problems regarding whooping cough in Iran and also low sensitivity of culturing method to diagnose Bordotella, complementary methods such as Real-Time PCR should be used.