FORMATION OF EOSINOPHILIC AND MONOCYTIC INTRADERMAL INFLAMMATORY SITES IN THE DOG BY INJECTION OF HUMAN RANTES BUT NOT HUMAN MONOCYTE CHEMOATTRACTANT PROTEIN-1, HUMAN MACROPHAGE INFLAMMATORY PROTEIN 1-ALPHA, OR HUMAN INTERLEUKIN-8
FORMATION OF EOSINOPHILIC AND MONOCYTIC INTRADERMAL INFLAMMATORY SITES IN THE DOG BY INJECTION OF HUMAN RANTES BUT NOT HUMAN MONOCYTE CHEMOATTRACTANT PROTEIN-1, HUMAN MACROPHAGE INFLAMMATORY PROTEIN 1-ALPHA, OR HUMAN INTERLEUKIN-8
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DOI：
10.1084/jem.178.6.1913
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发表时间：
1993-12-01
影响因子：
15.3
通讯作者：
ROSEN, H
中科院分区：
文献类型：
--
作者：
MEURER, R;VANRIPER, G;ROSEN, H
对犬单核细胞和粒细胞的平衡结合研究允许鉴定人C-C趋化因子RANTES的单一高亲和力受体（解离常数，14 +/- 8 pM），与人RANTES受体相反，其对人巨噬细胞炎性蛋白1 α（hMIP-1 α）没有亲和力。在犬中单次皮内注射hRANTES在4 h内导致嗜酸性粒细胞和巨噬细胞丰富的炎症部位。细胞浸润在hRANTES注射后16-24 h达到高峰。有组织学证据表明，在4小时的血管内激活的嗜酸性粒细胞，虽然在脉管系统和血小板中的嗜酸性粒细胞含有明显完整的颗粒。16-24 h，单核细胞是粘附于微静脉内皮的主要细胞。人MIP-1 α在犬真皮中未引起反应，而单核细胞趋化蛋白1引起单核细胞轻度血管周围套囊。与此相反，人白细胞介素8诱导的嗜酸性真皮浸润，是最大的4小时后的挑战。这提供了体内第一个直接证据，证明RANTES具有显著的促炎活性，此外，还可能是以嗜酸性粒细胞和单核细胞炎症反应为特征的特应性病理学中的介体。
Equilibrium binding studies on canine mononuclear and granulocytic cells allow the identification of a single high affinity receptor for the human C-C chemokine RANTES (dissociation constant, 14 +/- 8 pM), that, in contrast to the human RANTES receptor, has no affinity for human macrophage inflammatory protein 1alpha (hMIP-1alpha). A single intradermal injection of hRANTES in dog resulted in eosinophil- and macrophage-rich inflammatory sites within 4 h. Cell infiltration peaked at 16-24 h after hRANTES injection. There was histological evidence of intravascular activation of eosinophils at 4 h, although eosinophils in the vasculature and interstitium contained apparently intact granules. Monocytes were the predominant cells adherent to venular endothelium at 16-24 h. Human MIP-1alpha elicited no response in canine dermis, whereas monocyte chemoattractant protein 1 caused mild perivascular cuffing with monocytes. In contrast, human interleukin 8 induced a neutrophilic dermal infiltrate that was maximal by 4 h after challenge. This provides the first direct evidence in vivo that RANTES has significant proinflammatory activity and, in addition, could be a mediator in atopic pathologies characterized by eosinophilic and monocytic inflammatory responses.