Interactions of microorganisms isolated from gilthead sea bream, Sparus aurata L., on Vibrio harveyi, a pathogen of farmed Senegalese sole, Solea senegalensis (Kaup)
Interactions of microorganisms isolated from gilthead sea bream, Sparus aurata L., on Vibrio harveyi, a pathogen of farmed Senegalese sole, Solea senegalensis (Kaup)
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DOI：
10.1111/j.1365-2761.2005.00657.x
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发表时间：
2005-09-01
影响因子：
2.5
通讯作者：
Moriñigo, MA
中科院分区：
文献类型：
--
作者：
Chabrillón, M;Rico, RM;Moriñigo, MA
从养殖金头鲷（Sparus aurata）中分离到4株细菌，分别属于弧菌科（Vibrionaceae）、假单胞菌科（Pseudomonodaceae）和微球菌属（Micrococcus），研究了它们对养殖塞内加尔鳎（Solea senegalensis）皮肤和肠道粘液的粘附能力，以及对鳎病病原菌哈维氏弧菌（Vibrio harveyi）的拮抗作用。这些分离株对鞋底粘液的粘附性高于所测定的哈维氏弧菌的致病菌株。只有两个菌株对哈维氏弧菌有拮抗活性。在排斥、竞争和置换条件下，研究了4株分离物与哈维氏弧菌在皮肤和肠粘液粘附方面的相互作用。三个分离株能够减少附着到皮肤和肠道唯一的哈威氏弧菌的致病菌株的粘液在位移和排斥条件下，但在竞争条件下。通过口服给药，然后用致病性哈维氏弧菌菌株Lg 14/00攻击，评估分离物Pdp 11的体内益生菌潜力。一组50只塞内加尔比目鱼接受补充有10（8）cfu g（-1）冻干Lg 14/00的商业饲料15天。第二组鱼接受无补充的商业饮食。在攻击后，接受补充有潜在益生菌分离物的饮食的鱼的死亡率显著低于接受未补充的商业饮食的鱼。这项研究表明，干扰病原体附着的能力，以及对宿主表面的粘附，是选择用于培养塞内加尔比目鱼的候选益生菌的合适标准。
Four bacterial isolates from farmed gilthead sea bream, Sparus aurata, included in a previous study as members of the Vibrionaceae and Pseudomonodaceae and the genus Micrococcus, have been evaluated for their adhesive ability to skin and intestinal mucus of farmed Senegalese sole, Solea senegalensis, and their antagonistic effect on Vibrio harveyi, a pathogen of sole. These isolates showed higher adhesion to sole mucus than the pathogenic strains of V. harveyi assayed. Only two of the isolates showed antagonistic activity to V. harveyi. Interactions of the four isolates with V. harveyi in respect of adhesion to skin and intestinal mucus under exclusion, competition and displacement conditions were studied. Three isolates were able to reduce the attachment to skin and intestinal sole mucus of a pathogenic strain of V. harveyi under displacement and exclusion conditions, but not under competition conditions. The in vivo probiotic potential of isolate Pdp11 was assessed by oral administration followed by challenge with the pathogenic V. harveyi strain Lg14/00. A group of 50 Senegalese sole received a commercial diet supplemented with 10(8) cfu g(-1) of lyophilized Lg14/00 for 15 days. A second group of fish received a non-supplemented commercial diet. After challenge the mortality of the fish receiving the diet supplemented with the potential probiotic isolate was significantly lower than that in the fish receiving the non-supplemented commercial diet. This study has shown that the ability to interfere with attachment of pathogens, as well as the adhesion to host surfaces, are suitable criteria for selection of candidate probiotics for use in the culture of Senegalese sole.