A role for preadipocytes as macrophage-like cells
A role for preadipocytes as macrophage-like cells
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DOI：
10.1096/fasebj.13.2.305
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发表时间：
1999-02-01
期刊：
影响因子：
4.8
通讯作者：
Pénicaud, L
中科院分区：
文献类型：
--
作者：
Cousin, B;Munoz, O;Pénicaud, L
多项证据支持脂肪组织和免疫活性细胞之间的联系。这种联系在肥胖症中得到了体现，人类和遗传性肥胖的啮齿类动物都存在过度肥胖和免疫功能受损的情况。此外，前脂肪细胞和巨噬细胞都分泌许多参与炎症反应的因子。在这里，我们展示了细胞系和原代培养物中增殖的前脂肪细胞对微生物产生吞噬活性。这是通过吞噬作用测定和共聚焦显微镜证实的。当前脂肪细胞停止增殖并分化为脂肪细胞时，该功能消失。吞噬作用后，前脂肪细胞通过氧依赖性机制表现出杀菌活性。此外，前脂肪细胞和脂肪细胞均用单核细胞-巨噬细胞谱系标记 MOMA-2 染色，但特定成熟巨噬细胞标记（F4/80 和 Mac-1）呈阴性。这些结果表明前脂肪细胞可以起到巨噬细胞样细胞的作用，并提高了脂肪组织直接参与炎症过程的可能性。
Several lines of evidence have supported a link betweeen adipose tissue and immunocompetent cells. This link is illustrated in obesity, where excess adiposity and impaired immune function have been described in both humans and genetically obese rodents. In addition, numerous factors involved in inflammatory response are secreted by both preadipocytes and macrophages. Here we show that proliferating preadipocytes in cell lines and primary cultures, develop phagocytic activity toward microorganisms. This is demonstrated by phagocytosis assays and confocal microscopy. This function disappears when preadipocytes stop proliferating and differentiate into adipocytes. After phagocytosis, preadipocytes show microbicide activity via an oxygen-dependent mechanism. In addition, preadipocytes as well as adipocytes are stained with MOMA-2, a marker of monocyte-macrophage lineage, but are negative for specific mature macrophage markers (F4/80 and Mac-1). These results suggest that preadipocytes could function as macrophage-like cells and raise the possibility of a potential direct involvement of adipose tissue in inflammatory processes.