FASCICLIN-IV - SEQUENCE, EXPRESSION, AND FUNCTION DURING GROWTH CONE GUIDANCE IN THE GRASSHOPPER EMBRYO
FASCICLIN-IV - SEQUENCE, EXPRESSION, AND FUNCTION DURING GROWTH CONE GUIDANCE IN THE GRASSHOPPER EMBRYO
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DOI：
10.1016/0896-6273(92)90237-8
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发表时间：
1992-11-01
期刊：
影响因子：
16.2
通讯作者：
GOODMAN, CS
中科院分区：
文献类型：
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作者：
KOLODKIN, AL;MATTHES, DJ;GOODMAN, CS
用单抗6F8鉴定和克隆了蝗虫一种新的轴突糖蛋白Fasciclin IV，并研究了其在生长锥引导中的功能。Fasciclin IV在发育中的中枢神经系统轴突通路的一个子集上动态表达，在发育中的肢芽上皮细胞的周带上动态表达。其中一条带对应于Ti1前驱神经元的生长锥体向中枢神经系统延伸时发生特征性转弯的位置。在6F8抗体或Fab中培养的胚胎表现出这种轴突途径的异常形成。CDNA序列分析表明，Fasciclin IV具有信号序列；具有长的胞外、跨膜和短的细胞质结构域；并且与现有数据库中的任何蛋白质都没有同源性。因此，Fasciclin IV似乎是一种新的完整的膜蛋白，具有生长锥体导向功能。
Monoclonal antibody 6F8 was used to characterize and clone fasciclin IV, a new axonal glycoprotein in the grasshopper, and to study its function during growth cone guidance. Fasciclin IV is dynamically expressed on a subset of axon pathways in the developing CNS and on circumferential bands of epithelial cells in developing limb buds. One of these bands corresponds to the location where the growth cones of the Ti1 pioneer neurons make a characteristic turn while extending toward the CNS. Embryos cultured in the 6F8 antibody or Fab exhibit aberrant formation of this axon pathway. cDNA sequence analysis suggests that fasciclin IV has a signal sequence; long extracellular, transmembrane, and short cytoplasmic domains; and shows no homology with any protein in the available data bases. Thus, fasciclin IV appears to be a novel integral membrane protein that functions in growth cone guidance.