Supercritical CO2-assisted embossing for studying cell behaviour on microtextured surfaces
Supercritical CO2-assisted embossing for studying cell behaviour on microtextured surfaces
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DOI：
10.1016/j.biomaterials.2008.08.027
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发表时间：
2008-12-01
期刊：
影响因子：
14
通讯作者：
Iwata, Hiroo
中科院分区：
文献类型：
--
作者：
Fujita, Satoshi;Ono, Daizaburo;Iwata, Hiroo
近年来，细胞对微纳米结构的反应引起了人们的广泛关注。虽然已经观察到有趣的现象，但我们在阐明纯地形对细胞行为的影响方面遇到了一些困难。这些问题的部分原因是在表面加工过程中引入了官能团和化学品的持久性。在这项研究中，我们引入了超临界CO2辅助压花，通过溶解超临界CO2使聚碳酸酯板塑化，从而可以在低于聚碳酸酯玻璃化转变温度的温度下在板上压花大面积图案。在聚碳酸酯板表面的整个区域观察到均匀的微和纳米图案表面。制备的表面未检测到镍、氟和氮，表面碳氧比与根据聚碳酸酯分子结构计算的理论比（C:O = 84.2:15.8）相当。在制备的微晶状体和纳米沟槽基质上培养人间充质干细胞。微透镜上的细胞粘附面积比未处理的聚碳酸酯小。与此同时，细胞沿着山谷深度超过90纳米的纳米沟槽脊排列。这种超临界co2辅助压印可以为研究合成材料表面形貌对细胞行为的影响提供良好的衬底。(C) 2008 Elsevier Ltd版权所有。
Recently, cell responses to micro- and nanoscale structures have attracted much attention. Although interesting phenomena have been observed, we have encountered some difficulties in elucidating purely topographical effects on cell behaviour. These problems are partially attributable to the introduction of functional groups and the persistence of chemicals during surface processing. In this study, we introduced supercritical CO2-assisted embossing, which plasticizes a polycarbonate plate by dissolving supercritical CO2 and thus can emboss wide-scale patterns onto the plate at a lower temperature than the polycarbonate glass transition temperature. Uniform micro- and nanopatterned surfaces were observed across the whole area of the polycarbonate plate surfaces. Nickel, fluorine, and nitrogen were not detected on the fabricated surfaces, and the surface carbon-to-oxygen ratios were equivalent to the theoretical ratio (C:O = 84.2:15.8) calculated from the polycarbonate molecular structure. Human mesenchymal stem cells were cultured on the fabricated microlens and nanogroove substrata. Cell-adhered areas became smaller on the microlens than on non-treated polycarbonate. Meanwhile, cells aligned along the ridges of nanogrooves with valleys deeper than 90 nm. This supercritical CO2-assisted embossing can produce fine substrates for studying the effects of surface topography of synthetic materials on cell behaviours. (C) 2008 Elsevier Ltd. All rights reserved.