Assignment of the structural gene for subunit M1 of human ribonucleotide reductase to the short arm of chromosome 11.
Assignment of the structural gene for subunit M1 of human ribonucleotide reductase to the short arm of chromosome 11.
复制标题
人核糖核苷酸还原酶 M1 亚基的结构基因分配到 11 号染色体的短臂。
DOI：
10.1016/0014-4827(85)90470-7
复制
发表时间：
1985
影响因子：
3.7
通讯作者：
Francke,U
中科院分区：
文献类型：
--
作者：
Engström,Y;Francke,U
通过使用一个物种特异性的单克隆抗体，识别核糖核苷酸还原酶的亚基M1从人类，但不是仓鼠的起源，我们已经能够分配的结构基因为人类蛋白质M1的染色体11的短臂。从一组人-中国仓鼠体细胞杂种中提取的蛋白质在十二烷基硫酸钠（SDS）变性聚丙烯酰胺凝胶中进行电泳，然后转移并共价偶联到重氮苄氧基甲基纸上。通过与小鼠单克隆抗M1抗体AD 203孵育，然后与兔抗小鼠IgG、125 I标记的葡萄球菌蛋白A孵育，最后进行放射自显影，筛选人蛋白M1。在所有测试的杂交体中，人蛋白M1的检测与11号染色体的存在相关，特别是与该染色体的短臂相关。该区域还包含人类胰岛素基因、胰岛素样生长因子II基因和c-Harvey-ras1癌基因。
By using a species-specific monoclonal antibody that recognizes subunit M1 of ribonucleotide reductase from human but not hamster origin, we have been able to assign the structural gene for the human protein M1 to the short arm of chromosome 11. Protein extracts from a panel of human-Chinese hamster somatic cell hybrids were subjected to electrophoresis in sodium dodecyl sulfate (SDS) denaturating polyacrylamide gels, and then transferred and coupled covalently to diazobenzyloxymethyl paper. These were screened for human protein M1 by incubation with the mouse monoclonal anti-M1 antibody AD 203, followed by rabbit anti-mouse IgG,125I-labelledStaphylococcusprotein A and finally autoradiography. In all tested hybrids the detection of human protein M1 was correlated with the presence of chromosome 11, specifically with the short arm of this chromosome. This region also contains the human genes for insulin, insulin-like growth factor II, and the c-Harvey-ras1 oncogene.