Proteomics for the analysis of the Candida albicans biofilm lifestyle
Proteomics for the analysis of the Candida albicans biofilm lifestyle
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DOI：
10.1002/pmic.200600332
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发表时间：
2006-11-01
期刊：
影响因子：
3.4
通讯作者：
Lopez-Ribot, Jose L.
中科院分区：
文献类型：
--
作者：
Thomas, Derek P.;Bachmann, Stefano P.;Lopez-Ribot, Jose L.
白色念珠菌是一种机会致病真菌，能够引起免疫功能低下患者感染。寄生虫病通常与惰性或生物材料表面上的生物膜的形成有关。生物膜是附着在表面上的结构化微生物群落，并包裹在胞外聚合物（EPS）基质中。目前，很少有人知道的蛋白质谱的变化，发生在过渡过程中的反硝化生物膜生长模式。在这里，我们报告使用蛋白质组学的比较分析的亚细胞组分从C。白色念珠菌生物膜和渗透性培养物，包括细胞表面相关蛋白和液体培养物上清液（渗透性培养物）和EPS（生物膜）中存在的分泌组分。该分析揭示了高度的相似性之间的蛋白质谱与反渗透和生物膜提取物，并导致几个差异表达的蛋白质点的鉴定。在差异表达的蛋白质中，有一个优势的代谢酶，已被描述为细胞表面蛋白和免疫显性抗原。在生物膜基质中发现的蛋白质包括一些预测形成分泌组的一部分，以及许多无分泌信号的蛋白质。这些观察有助于我们对C.白色念珠菌生物膜生活方式。
Candida albicans is an opportunistic pathogenic fungus capable of causing infections in immunocompromised patients. Candidiasis is often associated with the formation of biofilms on the surface of inert or biological materials. Biofilms are structured microbial communities attached to a surface and encased within a matrix of exopolymeric substance (EPS). At present, very little is known about the changes in protein profiles that occur during the transition from the planktonic to the biofilm mode of growth. Here, we report the use of proteomics for the comparative analysis of subcellular fractions obtained from C. albicans biofilm and planktonic cultures, including cell surface-associated proteins and secreted components present in liquid culture supernatants (for planktonic cultures) and EPS (for biofilms). The analysis revealed a high degree of similarity between the protein profiles associated with the planktonic and biofilm extracts, and led to the identification of several differentially expressed protein spots. Among the differentially expressed proteins, there was a preponderance of metabolic enzymes that have been described as cell surface proteins and immunodominant antigens. Proteins found in the biofilm matrix included a few predicted to form part of the secretome, and also many secretion-signal-less proteins. These observations contribute to our understanding of the C. albicans biofilm lifestyle.