Increased expression of calcium-binding protein S100 in human uterine smooth muscle tumours
Increased expression of calcium-binding protein S100 in human uterine smooth muscle tumours
复制标题
DOI：
10.1093/molehr/gah100
复制
发表时间：
2004-10-01
影响因子：
4
通讯作者：
Fujii, S
中科院分区：
文献类型：
--
作者：
Kanamori, T;Takakura, K;Fujii, S
S100蛋白属于EF-hand Ca 2+结合蛋白家族，通过与不同靶蛋白的相互作用调节多种细胞过程。包括癌症和黑色素瘤在内的几种疾病与S100蛋白的异常表达有关，S100蛋白以细胞和组织特异性方式表达。我们研究了S100家族成员在人子宫平滑肌肿瘤中的表达。RT-PCR检测到S100蛋白家族的6个成员S100 A1、A4、A6、A7、A10和A11在人子宫平滑肌瘤和子宫肌层组织中表达，而其他成员未检测到表达。实时荧光定量PCR显示S100 A11在子宫肌瘤中的表达明显高于子宫肌层。小干扰RNA（siRNA）抑制S100 A11可导致细胞凋亡，S100 A11过表达可抑制人子宫平滑肌肿瘤细胞凋亡。提示S100 A11具有抗细胞凋亡的作用，与子宫肌瘤的生长过程有关。
S100 proteins belong to the EF-hand Ca2+ -binding protein family and regulate a variety of cellular processes via interaction with different target proteins. Several diseases, including cancer and melanoma, are related to the abnormal expression of S100 proteins, which are expressed in cell- and tissue-specific manners. We investigated the expression of S100 family members in human uterine smooth muscle tumours. Expression of six members of the S100 protein family: S100A1, A4, A6, A7, A10 and A11, was found in human uterine leiomyoma and myometrium tissue, but expression of other members was not detected by RT-PCR. Real-time PCR showed that S100A11 expression was significantly increased in leiomyoma compared with myometrium. Suppression of S100A11 by small interfering RNA (siRNA) led to apoptosis, and the overexpression of S100A11 inhibited apoptosis in human uterine smooth muscle tumour cells. These findings suggest that S100A11 has an anti-apoptotic function and is related to the process of growth of human uterine leiomyoma.