Cultivation conditions and the diffusion of oxygen into culture media: The rationale for the flask-to-medium ratio in microbiology
Cultivation conditions and the diffusion of oxygen into culture media: The rationale for the flask-to-medium ratio in microbiology
复制标题
DOI：
10.1186/1471-2180-13-9
复制
发表时间：
2013-01-16
期刊：
影响因子：
4.2
通讯作者：
Proctor, Richard A.
中科院分区：
文献类型：
--
作者：
Somerville, Greg A.;Proctor, Richard A.
细菌培养需要考虑三件事：细菌菌株、培养基和培养条件。大多数微生物学家在手稿中尽职尽责地报告他们对菌株和培养介质的选择；然而，这些微生物学家经常忽视报告培养条件。没有这些信息，就很难确定培养物是需氧培养、微需氧培养还是厌氧培养。为了好氧培养细菌，有必要了解氧气不容易扩散到培养基中；它需要帮助才能进入。微生物学家可以通过改变烧瓶与介质的比例、搅拌的转速和/或大气中氧气的浓度，或者使用有障板的烧瓶来做到这一点。
Bacterial cultivation requires consideration of three things: The bacterial strain, cultivation medium, and cultivation conditions. Most microbiologists dutifully report their choice of strains and cultivation media in manuscripts; however, these same microbiologists often overlook reporting cultivation conditions. Without this information, it is difficult to determine if cultures were grown aerobically, microaerobically, or anaerobically. To cultivate bacteria aerobically, it is necessary to understand that oxygen does not readily diffuse into culture media; it needs help to get in. Microbiologists can do this by altering the flask-to-medium ratio, rpm of agitation, and/or the concentration of atmospheric oxygen, or by using baffled flasks.