Non-invasive hydrodynamic imaging in plant roots at cellular resolution.

Non-invasive hydrodynamic imaging in plant roots at cellular resolution.
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DOI:
10.1038/s41467-021-24913-z
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发表时间:
2021-08-03
影响因子:
16.6
通讯作者:
Webb KF
Webb KF
中科院分区:
综合性期刊1区
文献类型:
--
作者:
Pascut FC;Couvreur V;Dietrich D;Leftley N;Reyt G;Boursiac Y;Calvo-Polanco M;Casimiro I;Maurel C;Salt DE;Draye X;Wells DM;Bennett MJ;Webb KF

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A key impediment to studying water-related mechanisms in plants is the inability to non-invasively image water fluxes in cells at high temporal and spatial resolution. Here, we report that Raman microspectroscopy, complemented by hydrodynamic modelling, can achieve this goal - monitoring hydrodynamics within living root tissues at cell- and sub-second-scale resolutions. Raman imaging of water-transporting xylem vessels in Arabidopsis thaliana mutant roots reveals faster xylem water transport in endodermal diffusion barrier mutants. Furthermore, transverse line scans across the root suggest water transported via the root xylem does not re-enter outer root tissues nor the surrounding soil when en-route to shoot tissues if endodermal diffusion barriers are intact, thereby separating ‘two water worlds’. Existing methods for non-invasively monitoring water flow in plants have limited spatial/temporal resolution. Here, the authors report that Raman microspectroscopy, complemented by hydrodynamic modelling, can monitor hydrodynamics within living root tissues at cell- and sub-second-scale resolutions.
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