An Elastomeric Patch Electrospun from a Blended Solution of Dermal Extracellular Matrix and Biodegradable Polyurethane for Rat Abdominal Wall Repair
An Elastomeric Patch Electrospun from a Blended Solution of Dermal Extracellular Matrix and Biodegradable Polyurethane for Rat Abdominal Wall Repair
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DOI：
10.1089/ten.tec.2011.0295
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发表时间：
2012-02-01
影响因子：
3
通讯作者：
Wagner, William R.
中科院分区：
文献类型：
--
作者：
Hong, Yi;Takanari, Keisuke;Wagner, William R.
通过静电纺丝聚（酯氨基甲酸酯）脲（PEUU）和猪真皮细胞外基质（dECM）消化物的混合溶液来产生可生物降解的弹性体支架，其中包括PEUU以提供弹性、柔性和机械支撑，并且dECM用于增强生物活性和生物相容性。显微照片和差示扫描量热法证明PEUU和dECM之间的部分相容性。随着更大的dECM含量，支架被发现具有较低的断裂应变和缝线保持强度，虽然初始模量是更高的dECM浓度。含有0%至50%dECM的杂化支架的拉伸强度为5至7MPa，断裂应变为138%至611%，初始模量为3至11MPa，缝线保持强度为35至59MPa。当水合时，发现支架在50%dECM含量下显著收缩。当用于大鼠全层腹壁置换模型时，使用含有25%dECM的支架或对照100%PEUU支架4周和8周后未观察到疝、感染或组织粘连。结合dECM的支架在外植体时显著更厚，与对照相比具有更多数量的相关平滑肌肌动蛋白阳性染色细胞，但无论dECM添加如何，都检测到支架的最小细胞浸润和重塑。因此，从混合溶液中加工dECM和PEUU提供了具有更好生物活性的证据和单独消化的dECM无法实现的机械性质的支架。
A biodegradable elastomeric scaffold was created by electrospinning a mixed solution of poly(ester urethane)urea (PEUU) and porcine dermal extracellular matrix (dECM) digest, with PEUU included to provide elasticity, flexibility, and mechanical support and dECM used to enhance bioactivity and biocompatibility. Micrographs and differential scanning calorimetry demonstrated partial miscibility between PEUU and dECM. With greater dECM content, scaffolds were found to possess lower breaking strains and suture retention strength, although initial modulus was greater with higher dECM concentrations. The hybrid scaffolds containing 0% to 50% dECM had tensile strengths of 5 to 7 MPa, breaking strains of 138% to 611%, initial moduli of 3 to 11 Mpa, and suture retention strengths of 35 to 59 MPa. When hydrated, scaffolds were found to contract markedly with 50% dECM content. When used in a rat full-thickness abdominal wall replacement model, no herniation, infection, or tissue adhesion was observed after 4 and 8 weeks with a scaffold containing 25% dECM or a control 100% PEUU scaffold. Scaffolds incorporating dECM were significantly thicker at the time of explant, with greater numbers of associated smooth muscle actin positive staining cells than in the control, but minimal cellular infiltration and remodeling of the scaffold were detected regardless of dECM addition. The processing of dECM and PEUU from a mixed solution thus provided a scaffold with evidence of better bioactivity and with mechanical properties not achievable with digested dECM alone.