Conserved structures of mediator and RNA polymerase II holoenzyme
Conserved structures of mediator and RNA polymerase II holoenzyme
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DOI：
10.1126/science.283.5404.985
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发表时间：
1999-02-12
期刊：
影响因子：
56.9
通讯作者：
Kornberg, RD
中科院分区：
文献类型：
--
作者：
Asturias, FJ;Jiang, YW;Kornberg, RD
通过电子显微镜和图像处理揭示了转录调节介质（介质）和RNA聚合酶II全酶的单个颗粒。介质单独出现紧凑，但在高pH值或在RNA聚合酶II的存在下，它显示出一个扩展的构象。全酶含有完全伸展状态的介体，部分包裹球状聚合酶，在聚合酶羧基末端结构域和DNA结合通道附近具有明显接触点。酵母和鼠复合物在外观和构象行为上的相似性表明真核生物中的介体结构是保守的。
Single particles of the mediator of transcriptional regulation (Mediator) and of RNA polymerase II holoenzyme were revealed by electron microscopy and image processing. Mediator alone appeared compact, but at high pH or in the presence of RNA polymerase II it displayed an extended conformation. Holoenzyme contained Mediator in a fully extended state, partially enveloping the globular polymerase, with points of apparent contact in the vicinity of the polymerase carboxyl-terminal domain and the DNA-binding channel. A similarity in appearance and conformational behavior of yeast and murine complexes indicates a conservation of Mediator structure among eukaryotes.