Poly(3,4-ethylenedioxythiophene)/poly(styrenesulfonate)-poly(vinyl alcohol)/poly(acrylic acid) interpenetrating polymer networks for improving optrode-neural tissue interface in optogenetics
Poly(3,4-ethylenedioxythiophene)/poly(styrenesulfonate)-poly(vinyl alcohol)/poly(acrylic acid) interpenetrating polymer networks for improving optrode-neural tissue interface in optogenetics
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聚(3,4-乙撑二氧噻吩)/聚(苯乙烯磺酸)-聚(乙烯醇)/聚(丙烯酸)互穿聚合物网络用于改善光遗传学中的光极-神经组织界面
DOI：
10.1016/j.biomaterials.2011.09.083
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发表时间：
2012-01-01
期刊：
影响因子：
14
通讯作者：
Wang, Liping
中科院分区：
文献类型：
--
作者：
Lu, Yi;Li, Yanling;Wang, Liping
光遗传学领域已经成功地通过对神经回路中特定神经元组的精确空间和时间控制来理解神经精神疾病的机制。然而，将光遗传调制与电生理和行为读出方法相结合，作为一种手段来探索自由行为动物特定回路中神经元之间的因果、时间上的精确和行为相关的相互作用，仍然是一个巨大的挑战。在这项研究中，我们制作了一个八通道的可长期植入的光学电极阵列，并用poly(3，4-ethylenedioxythiophene)/poly(styrenesulfonate)-poly(vinyl/PSS-PVA/PAA互穿聚合物网络(PEDOT/PSS-PVA/PAA IPN)对其进行了修饰，以改善光学电极-神经组织的界面。导电聚合物-水凝胶IPN薄膜在1 kHz下表现出比未修饰的光电极位更高的电容和更低的电化学阻抗，并且与纯导电聚合物薄膜相比具有更好的机械稳定性和电化学稳定性。钙黄素-AM染色可清楚地观察到大鼠嗜铬细胞瘤(PC12)细胞在IPN膜上的细胞附着和突起生长。此外，在慢病毒表达突触素-ChR2-EYFP后，将光电极阵列慢性植入SD大鼠的海马区，在自由活动的动物中获得光诱发的频率依赖的动作电位。电记录结果表明，与未修饰的位置相比，修饰后的光电极阵列的阻抗和均方根噪声显著降低，信噪比提高，这可能得益于沉积的IPN薄膜的电化学性能和生物相容性的改善。所有这些特性在光遗传应用中都是非常需要的，导电聚合物-水凝胶互穿网络的制备方法可以很容易地与其他修饰方法相结合，构建更先进的光学电极-神经组织界面。(C)2011爱思唯尔有限公司。保留所有权利。
The field of optogenetics has been successfully used to understand the mechanisms of neuropsychiatric diseases through the precise spatial and temporal control of specific groups of neurons in a neural circuitry. However, it remains a great challenge to integrate optogenetic modulation with electrophysiological and behavioral read out methods as a means to explore the causal, temporally precise, and behaviorally relevant interactions of neurons in the specific circuits of freely behaving animals. In this study, an eight-channel chronically implantable optrode array was fabricated and modified with poly(3,4-ethylenedioxythiophene)/poly(styrenesulfonate)-poly(vinyl alcohol)/poly(acrylic acid) interpenetrating polymer networks (PEDOT/PSS-PVA/PAA IPNs) for improving the optrode-neural tissue interface. The conducting polymer-hydrogel IPN films exhibited a significantly higher capacitance and lower electrochemical impedance at 1 kHz as compared to unmodified optrode sites and showed significantly improved mechanical and electrochemical stability as compared to pure conducting polymer films. The cell attachment and neurite outgrowth of rat pheochromocytoma (PC12) cells on the IPN films were clearly observed through calcein-AM staining. Furthermore, the optrode arrays were chronically implanted into the hippocampus of SD rats after the lentiviral expression of synapsin-ChR2-EYFP, and light-evoked, frequency-dependant action potentials were obtained in freely moving animals. The electrical recording results suggested that the modified optrode arrays showed significantly reduced impedance and RMS noise and an improved SNR as compared to unmodified sites, which may have benefited from the improved electrochemical performance and biocompatibility of the deposited IPN films. All these characteristics are greatly desired in optogenetic applications, and the fabrication method of conducting polymer-hydrogel IPNs can be easily integrated with other modification methods to build a more advanced optrode-neural tissue interface. (C) 2011 Elsevier Ltd. All rights reserved.