Developmental hemostasis: age-specific differences in the levels of hemostatic proteins
Developmental hemostasis: age-specific differences in the levels of hemostatic proteins
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DOI：
10.1111/jth.12372
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发表时间：
2013-10-01
影响因子：
10.4
通讯作者：
Ignjatovic, V.
中科院分区：
文献类型：
--
作者：
Attard, C.;van der Straaten, T.;Ignjatovic, V.
发育性止血认识到新生儿、儿童和成人止血系统的生理差异。与成人的止血系统生理学知识相比，我们对新生儿和儿童的了解仍然不足。常规临床凝血试验最常测量止血系统的功能参数。很少有研究测量了止血蛋白的年龄特异性水平。了解止血蛋白水平的正常波动对于预防、诊断和治疗婴儿期和儿童期的止血问题至关重要。本研究是首次对健康新生儿、儿童和成人中重要止血蛋白水平的年龄特异性变化进行综合研究。方法选取新生儿（第1天和第3天）、28天至1岁、1-5岁、6-10岁、11-16岁和成人共120例健康人的血浆样本。采用市售ELISA试剂盒定量FactorII、FV、FVII、FVIII、FIX、FX、FXI、FXII、FXIII、纤溶酶原、proteinC、总蛋白和游离蛋白。结果10种蛋白的水平在新生儿和成人之间存在显著差异，并且这些差异在整个儿童时期持续存在。结论多数凝血蛋白水平随年龄变化明显。未来的研究应探讨止血蛋白水平与功能变化随年龄的关系。
IntroductionDevelopmental hemostasis recognizes the physiologic differences between the hemostatic system of neonates and children and that of adults. As compared with the knowledge of hemostatic system physiology in adults, our understanding in neonates and children remains inadequate. Routine clinical coagulation testing most commonly measures functional parameters of the hemostatic system. Very few studies have measured age-specific levels of hemostatic proteins. An understanding of the normal fluctuations in the levels of hemostatic proteins is vital in the prevention, diagnosis and treatment of hemostatic problems during infancy and childhood. This study was designed as the first comprehensive study of the age-specific changes in the levels of important hemostatic proteins in healthy neonates, children, and adults.MethodsPlasma samples were obtained from 120 healthy individuals from the following age groups: neonates (day1 and day3), 28days to 1year, 1-5years, 6-10years, 11-16years, and adults. FactorII, FV, FVII, FVIII, FIX, FX, FXI, FXII, FXIII, plasminogen, proteinC and total and free proteinS were quantified with commercially available ELISA kits.ResultsThe levels of 10 proteins were significantly different between neonates and adults, and these differences persisted throughout childhood for most of these proteins.ConclusionThe results of this study confirm that the levels of the majority of coagulation proteins vary significantly with age. Future studies should investigate how hemostatic protein level relates to functional changes with age.