Protective effects of hesperidin on concanavalin A-induced hepatic injury in mice
Protective effects of hesperidin on concanavalin A-induced hepatic injury in mice
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DOI：
10.1016/j.intimp.2014.05.018
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发表时间：
2014-08-01
影响因子：
5.6
通讯作者：
Tang, Shao-qian
中科院分区：
文献类型：
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作者：
Li, Gang;Chen, Mao-jian;Tang, Shao-qian
橙皮苷（Hesperidin，HDN）是一种广泛存在于多种植物中的柑橘类生物碱。HDN具有抗氧化、抗肿瘤、抗炎、免疫调节等作用，本研究探讨HDN对刀豆蛋白A（ConA）诱导的肝损伤的保护作用。采用ConA（15 mg/kg）静脉注射建立C57 BL/6小鼠急性肝损伤模型，并在ConA攻击前给予HDN预处理10 d。结果发现，HDN预处理小鼠的肝损伤明显改善。此外，肝氧化应激和促炎细胞因子包括TNF-α的产生。HDN预处理可降低IFN-γ的表达。更重要的是，与ConA处理的小鼠相比，HDN预处理的小鼠的HMGB 1的表达和释放以及T细胞活化显著降低。因此，这些结果表明，HDN通过抑制肝细胞氧化应激，产生细胞因子，表达和释放HMGB 1和活化T细胞来保护小鼠免受Con A诱导的肝损伤。(C)2014爱思唯尔有限公司版权所有。
Hesperidin (HDN) is a citrus bioflavonoid, which widely exists in many plants. Previous researches have proved that HDN has several functions such as anti-oxidant, anti-tumor, anti-inflammatory, immune regulation and so on. In the present study, we explored the protective effects of HDN on concanavalin A (Con A)-induced hepatic injury. Acute hepatic injury model was established successfully by intravenous administration of Con A (15 mg/kg) in male C57BL/6 mice, and HDN was pretreated for 10 days before Con A challenge. It was found that the hepatic injury was notably improved in HDN pretreated mice. Furthermore, hepatic oxidative stress and the production of proinflammatory cytoldnes including TNF-alpha. and IFN-gamma were decreased by HDN pretreatment. More importantly, compared with Con A-treated mice, the expression and releasing of HMGB1 and T-cell activation were markedly reduced in HDN pretreated mice. Thus, these results suggest that HDN protects mice from Con A-induced hepatic injury by suppressing hepatocyte oxidative stress, producing cytokines, expressing and releasing HMGB1 and activating T cells. (C) 2014 Elsevier B.V. All rights reserved.