QUANTITATIVE-DETERMINATION OF GLUTAMATE MEDIATED CORTICAL NEURONAL INJURY IN CELL-CULTURE BY LACTATE-DEHYDROGENASE EFFLUX ASSAY
QUANTITATIVE-DETERMINATION OF GLUTAMATE MEDIATED CORTICAL NEURONAL INJURY IN CELL-CULTURE BY LACTATE-DEHYDROGENASE EFFLUX ASSAY
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DOI：
10.1016/0165-0270(87)90041-0
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发表时间：
1987-05-01
影响因子：
3
通讯作者：
CHOI, DW
中科院分区：
文献类型：
--
作者：
KOH, JY;CHOI, DW
已发现测量释放到细胞外浴介质的乳酸脱氢酶（LDH）活性是用于评估皮质细胞培养中谷氨酸介导的中枢神经元细胞损伤的简单而定量的方法。细胞外LDH在化学和生物学上都是稳定的;培养物中LDH流出量的大小与谷氨酸暴露损伤的神经元数量呈线性相关。
Measurement of lactate-dehydrogenase (LDH) activity released to the extracellular bathing media has been found to be a simple yet quantitative method for assessing glutamate mediated central neuronal cell injury in cortical cell culture. Extracellular LDH is both chemically and biologically stable; the magnitude of LDH efflux in the cultures correlates in a linear fashion with the number of neurons damaged by glutamate exposure.