Phenotypic characterization of an international Pseudomonas aeruginosa reference panel: strains of cystic fibrosis (CF) origin show less in vivo virulence than non-CF strains
Phenotypic characterization of an international Pseudomonas aeruginosa reference panel: strains of cystic fibrosis (CF) origin show less in vivo virulence than non-CF strains
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DOI：
10.1099/mic.0.000155
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发表时间：
2015-10-01
期刊：
影响因子：
2.8
通讯作者：
McClean, Siobhan
中科院分区：
文献类型：
--
作者：
Cullen, Louise;Weiser, Rebecca;McClean, Siobhan
铜绿假单胞菌引起囊性纤维化（CF）患者的慢性肺部感染和非CF患者的急性机会性感染。来自一系列临床和环境来源的42株铜绿假单胞菌菌株被整理成一个单一的参考菌株小组，以协调对这种多样化机会性病原体的研究。为了进一步协调和比较铜绿假单胞菌的研究，我们对面板菌株的生长速度、运动能力、毒力、花青素和海藻酸盐的产生、粘液表型、LPS模式、生物膜形成、脲酶活性、抗菌和噬菌体敏感性进行了表征。在铜绿假单胞菌组中，所有表型的表型多样性都很明显，这与在该物种中观察到的显著变异性一致。然而，除了生长速度外，CF源菌株与非CF源菌株之间的表型多样性具有可比性。CF菌株的毒力低于非CF菌株（P=0.037）。传染性CF菌株一般缺乏o抗原，产生较少的pyocyanin，对大蜡杆菌的毒力较低。此外，在三组序列CF菌株中，较早分离的菌株毒力、o抗原表达和pyocyanin产量均高于较晚感染的菌株。总的来说，铜绿假单胞菌菌株定义组的完整表型特征增加了我们对铜绿假单胞菌毒力和发病机制的理解，并可能为针对这一问题病原体的新疗法的测试提供宝贵的资源。
Pseudomonas aeruginosa causes chronic lung infections in people with cystic fibrosis (CF) and acute opportunistic infections in people without CF. Forty-two P. aeruginosa strains from a range of clinical and environmental sources were collated into a single reference strain panel to harmonise research on this diverse opportunistic pathogen. To facilitate further harmonized and comparable research on P. aeruginosa, we characterized the panel strains for growth rates, motility, virulence in the Galleria mellonella infection model, pyocyanin and alginate production, mucoid phenotype, LPS pattern, biofilm formation, urease activity, and antimicrobial and phage susceptibilities. Phenotypic diversity across the P. aeruginosa panel was apparent for all phenotypes examined, agreeing with the marked variability seen in this species. However, except for growth rate, the phenotypic diversity among strains from CF versus non-CF sources was comparable. CF strains were less virulent in the G. mellonella model than non-CF strains (P=0.037). Transmissible CF strains generally lacked O-antigen, produced less pyocyanin and had low virulence in G. mellonella. Furthermore, in the three sets of sequential CF strains, virulence, O-antigen expression and pyocyanin production were higher in the earlier isolate compared to the isolate obtained later in infection. Overall, this full phenotypic characterization of the defined panel of P. aeruginosa strains increases our understanding of the virulence and pathogenesis of P. aeruginosa and may provide a valuable resource for the testing of novel therapies against this problematic pathogen.