Phenotyping of transgenic cloned piglets
Phenotyping of transgenic cloned piglets
复制标题
DOI：
10.1089/153623002320253319
复制
发表时间：
2002-01-01
期刊：
影响因子：
--
通讯作者：
Prather, Randall S.
中科院分区：
文献类型：
--
作者：
Carter, D. Bart;Lai, Liangxue;Prather, Randall S.
许多报告列举了克隆绵羊和牛所获得的异常现象。迄今为止，很少有报告提供关于克隆动物的总体健康状况和性能数据的详细信息。本报告跟踪了三窝共10只转基因克隆仔猪从出生到青春期的发育过程。包括体格检查的显著结果和对给药的反应，以及研究期间死亡的5头仔猪的尸检数据。该组仔猪的出生体重、胎盘重量和生长速率与在相同环境中饲养的对照动物无差异。在2日龄和2、4、8、12、16、20和24周龄时收集血液学和血清化学数据。结果表明，轻度贫血和低蛋白血症的克隆仔猪从出生到4周龄，但这两种情况都纠正了8周龄。对7头仔猪进行超声心动图检查。未检测到解剖缺陷，但其中3头仔猪的心输出量值降低。然而，这两只动物都在生长，没有临床疾病的证据。通过定量血清IgM和IgG水平以及通过测定B细胞、巨噬细胞、辅助T细胞（CD 4）、细胞毒性T细胞（CD 8）和双阳性T细胞（CD 4/CD 8）的数量来评价免疫系统。除一只动物外，未检测到受检仔猪免疫系统异常。在本研究过程中，10只仔猪中有5只被安乐死或死亡，表明克隆仔猪的死亡率很高，但其余5只克隆仔猪没有可检测到的缺陷。
Numerous reports list the abnormalities obtained from cloning sheep and cattle. To date, few reports provide detailed information regarding the overall health status and performance data of cloned animals. This report follows three litters totaling 10 transgenic cloned piglets from birth through puberty. Significant findings from physical examinations and response to treatments are included, as well as necropsy data from five of the piglets that died during the study. The birth weights, placental weights, and growth rates for this group of piglets were not different from that of control animals raised in the same environment. Hematology and serum chemistry data were collected at 2 days of age, and at 2, 4, 8, 12, 16, 20, and 24 weeks of age. Results indicated a mild anemia and hypoproteinemia in the cloned piglets from birth through 4 weeks of age, but both conditions were corrected by 8 weeks of age. Echocardiography was performed on seven of the piglets. No anatomical defects were detected, but three of the piglets had decreased cardiac output values. However, both animals are growing and show no evidence of clinical disease. The immune system was evaluated by quantification of serum IgM and IgG levels and by determining the population of B-cells, macrophages, helper T-cells (CD4), cytotoxic T-cell (CD8), and double positive T-cells (CD4/CD8). With the exception of one animal, no abnormalities were detected with the immune system of the examined piglets. During the course of this study, five of the 10 piglets were euthanized or died, indicating there is a high mortality rate among cloned piglets, but the remaining five cloned piglets are free from detectable defects.