The human liver glutathione S-transferase gene superfamily: expression and chromosome mapping of an Hb subunit cDNA.
The human liver glutathione S-transferase gene superfamily: expression and chromosome mapping of an Hb subunit cDNA.
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人肝谷胱甘肽 S-转移酶基因超家族：Hb 亚基 cDNA 的表达和染色体作图。
DOI：
10.1093/nar/16.17.8541
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发表时间：
1988
影响因子：
14.9
通讯作者：
Tu,CP
中科院分区：
文献类型：
--
作者：
DeJong,JL;Chang,CM;Whang-Peng,J;Knutsen,T;Tu,CP
我们从λ gt 10 cDNA文库中分离到一个克隆λ GTH 4，它编码人肝谷胱甘肽S-转移酶Hb亚基，命名为亚基4。在E.随后的纯化和免疫印迹分析提供了结构和功能关系的明确归属。与人肝poly（A）RNA杂交显示一条约1200个核苷酸的条带，其大小与大鼠脑Yb 3 mRNA相当。亚基4相对于四种大鼠Yb亚基的氨基酸替代位点的差异分析表明，它与脑特异性Yb 3亚基关系最密切。λ GTH 4与Yb 3cDNA的3′端非翻译区核苷酸序列同源性进一步证实了这一结论，原位染色体定位将该谷胱甘肽S-转移酶基因定位于1号染色体p31区域。包括我们在内的许多实验室的结果表明，人类谷胱甘肽S-转移酶由一个基因超家族编码，该基因超家族位于至少两条不同的染色体上。
We have isolated from a λgtl0 cDNA library a clone λGTH4 which encodes a human liver glutathione S-transferase Hbsubunit, designated as subunit 4. Expression of this cDNA inE. coliand subsequent purification and immunoblotting analysis provided a definitive assignment of a structure and function relationship. RNA blot hybridization with human liver poly(A) RNA revealed a single band of ∼1200 nucleotides, comparable in size to the rat brain Yb3mRNA. Divergence analysis of amino acid replacement sites in subunit 4 relative to the four rat Ybsubunits revealed that it is most closely related to the brain-specific Yb3subunit. This conclusion is further substantiated by the nucleotide sequence homology between λGTH4 and the Yb3cDNA in their 3′ untranslated region.In situchromosome mapping has located this glutathione S-transferase gene in the region of p31 on chromosome 1. Results from many laboratories, including ours, indicate that the human glutathione S-transferases are encoded by a gene superfamily which is located on at least two different chromosomes.