Tissue levels of luteinizing hormone-releasing hormone in the preoptic area and hypothalamus, and serum concentrations of gonadotropins following anterior hypothalamic deafferentation and estrogen treatment of the female rat.
Tissue levels of luteinizing hormone-releasing hormone in the preoptic area and hypothalamus, and serum concentrations of gonadotropins following anterior hypothalamic deafferentation and estrogen treatment of the female rat.
复制标题
雌性大鼠下丘脑前部传入神经阻滞和雌激素治疗后，视前区和下丘脑的黄体生成素释放激素的组织水平以及促性腺激素的血清浓度。
DOI：
10.1210/endo-99-1-101
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发表时间：
1976
期刊：
影响因子：
4.8
通讯作者：
S. Kalra
中科院分区：
文献类型：
--
作者：
S. Kalra
雌性大鼠切除卵巢前下丘脑前去传入（AHD）或假AHD的内侧基底下丘脑（MBH）。在实验1和实验2中，在断头后30天和28天结束时测量MBH和视前区（POA）中的LHRH以及LHRH、FSH和LH的血液浓度。MBH前神经连接的中断导致MBH中LHRH的量急剧减少，POA显著增加。FSH（实验1和2）和LH（实验2）的循环水平显着抑制，而血清LHRH是不变的deafferentation。在实验3中，大鼠在AHD或假AHD后25天进行卵巢切除，并在卵巢切除后第7天给予苯甲酸雌二醇（EB，10 μ g/大鼠油，SC）或单独给予油，并在2天后处死。相对于假AHD，在AHD大鼠中观察到MBH LHRH的减少和POA LHRH的显著升高。血清LH和FSH浓度也显著降低。EB治疗显着降低血清促性腺激素，而LHRH水平在MBH的AHD和假AHD大鼠增加了两倍。EB注射后AHD大鼠血清LHRH浓度也显著降低。这些研究表明：1）由于在雌性大鼠MBH中正常检测到的LHRH活性的很大一部分似乎来自于吻侧区合成的LHRH活性，因此推断POA LHRH可能参与LH的紧张性和周期性放电是合乎逻辑的，和2）雌激素处理后MBH中LHRH含量的增加可能是由于MBH中LHRH释放的部分抑制和/或合成速率的增加。
Female rats were ovariectomized prior to anterior hypothalamic deafferentation (AHD) or sham AHD of the medial basal hypothalamus (MBH). LHRH in the MBH and the preoptic area (POA), and the blood concentrations of LHRH, FSH, and LH were measured following decapitation at the end of 30 days in experiment 1 and 28 days in experiment 2. Interruption of anterior neural links of the MBH resulted in a drastic reduction in the amounts of LHRH in the MBH and a significant increment in the POA. The circulating levels of FSH (experiments 1 and 2) and LH (experiment 2) were significantly depressed whereas serum LHRH was unaltered following deafferentation. In experiment 3, rats were ovariectomized 25 days following either AHD or sham AHD, and were given estradiol benzoate (EB, 10 mug/rat in oil, SC) or oil alone on day 7 postovariectomy and sacrificed 2 days later. Relative to sham AHD, a reduction in the MBH LHRH and a pronounced elevation in the POA LHRH was observed in AHD rats. Serum LH and FSH concentrations were also significantly decreased. EB treatment significantly lowered serum gonadotropins, whereas LHRH levels in the MBH of both AHD and sham AHD rats increased two-fold. Serum LHRH concentrations were also significantly reduced in AHD rats following EB injection. These studies indicate that 1) since a substantial portion of the LHRH activity normally detected in the MBH of female rats appears to be derived from that synthesized in the rostral regions, it is logical to infer that POA LHRH may be involved in the tonic as well as the cyclic discharge of LH, and 2) an increase in the LHRH content of the MBH after estrogen treatment may be due to a partial inhibition in release and/or an increased rate of synthesis of LHRH in the MBH.