Improved diagnostic accuracy using monoclonal antibody group A streptococcal carbohydrate.
Improved diagnostic accuracy using monoclonal antibody group A streptococcal carbohydrate.
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使用 A 组链球菌碳水化合物单克隆抗体提高了诊断准确性。
DOI：
10.1128/jcm.12.4.506-508.1980
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发表时间：
1980
影响因子：
9.4
通讯作者：
Davie,JM
中科院分区：
文献类型：
--
作者：
Nahm,MH;Murray,PR;Clevinger,BL;Davie,JM
在诊断微生物学实验室对抗A组链球菌糖链的鼠单克隆抗体进行了评价。用免疫荧光法和兰斯菲尔德沉淀试验对262株β-溶血性链球菌进行分类。非克隆抗体和商品化抗体均通过沉淀素试验鉴定为反应性抗体，但通过免疫荧光检测具有显著差异。单克隆抗体的反应性与沉淀素试验相同，但商业抗体的假阳性和假阴性结果都有。这些结果表明，单克隆试剂可能是上级的常规抗血清。
Monoclonal mouse antibody to streptococcal group A carbohydrate was evaluated in diagnostic microbiology laboratory. A total of 262 isolates of beta-hemolytic streptococci were classified with commercial reagents and the monoclonal antibody to group A carbohydrate by using immunofluorescence and Lancefield precipitin tests. Both nonoclonal and commercial antibodies were identifically reactive by the precipitin test but significantly different by immunofluroescence. Monoclonal antibody gave the same reactivity as seen in the precipitin test, but commercial antibodies gave both false-positive and false-negative results. These results suggest that monoclonal reagents may be superior to conventional antisera.