Mass Production of the Entomogenous Nematode Heterorhabditis heliothidis (Nematoda: Heterorhabditidae) on Artificial Media.
Mass Production of the Entomogenous Nematode Heterorhabditis heliothidis (Nematoda: Heterorhabditidae) on Artificial Media.
复制标题
在人工培养基上大规模生产虫生线虫Heterorhabditis heliothidis（线虫纲：Heterorhabditidae）。
作者：
W. M. Wouts
异habditis heliothidis在由市售营养肉汤、酵母提取物和植物油组成的人工培养基上单性饲养。这些成分用面粉煮熟，涂在聚醚聚氨酯海绵上，高压灭菌，用线虫细菌共生体悬浮液接种，在25℃下孵育3天。海绵上的细菌花园提供了一个很好的饲养培养基。每250毫升饲养瓶在一个月内可产生多达1000万具传染性的幼鱼。
Heterorhabditis heliothidis is reared monoxenically on an artificial medium consisting of commercially available nutrient broth, yeast extract, and vegetable oil. These components are cooked with flour and coated onto polyether polyurethane sponge, autoclaved, inoculated with a suspension of the bacterial symbiont of the nematode, and incubated at 25 C for 3 d. The bacterial garden on sponge provides an excellent rearing medium. Up to 10 million infective juveniles are produced per 250 ml rearing flask in one month.