Comparable response of ccn1 with ccn2 genes upon arthritis: An in vitro evaluation with a human chondrocytic cell line stimulated by a set of cytokines.
Comparable response of ccn1 with ccn2 genes upon arthritis: An in vitro evaluation with a human chondrocytic cell line stimulated by a set of cytokines.
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DOI：
10.1186/1478-811x-3-6
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发表时间：
2005-04-15
期刊：
影响因子：
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通讯作者：
Takigawa M
中科院分区：
文献类型：
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作者：
Moritani NH;Kubota S;Sugahara T;Takigawa M
软骨肉瘤来源的HCS-2/8细胞已被认为是人类关节软骨细胞的优秀模型。通过用细胞因子处理HCS-2/8细胞模拟关节炎症条件，我们在体外估计了CCN1和CCN2在关节炎症过程中的基因表达反应。为了模拟炎症的启动过程，用肿瘤坏死因子-α处理HcS-2/8细胞。为了诱导促炎或修复反应，使用转化生长因子-β。一种抗炎糖皮质激素的效果也被评估。刺激后，定量分析CCN1和CCN2的表达水平。令人惊讶的是，不仅ccn2和ccn1的表达在肿瘤坏死因子-α刺激下受到抑制，而这两种mRNAs都被转化生长因子-β和糖皮质激素统一诱导。这些结果描述了炎症过程中的相同反应，表明这两个CCN家族成员在关节炎过程中扮演着相似和合作的角色。
The chondrosarcoma-derived HCS-2/8 has been known to be an excellent model of human articular chondrocytes. By mimicking the arthritic conditions through the treatment of HCS-2/8 cells with cytokines, we estimated the gene expression response of ccn1 and ccn2 during the course of joint inflammation in vitro. In order to mimic the initiation of inflammation, HCS-2/8 cells were treated with tumor necrosis factor (TNF)-α. To induce pro-inflammatory or reparative responses, TGF-β was employed. Effects of an anti-inflammatory glucocorticoid were also evaluated. After stimulation, expression levels of ccn1 and ccn2 were quantitatively analyzed. Surprisingly, not only ccn2, but also ccn1 expression was repressed upon TNF-α stimulation, whereas both mRNAs were uniformly induced by transforming growth factor (TGF)-β and a glucocorticoid. These results describing the same response during the course of inflammation suggest similar and co-operative roles of these 2 ccn family members in the course of arthritis.