Multi-layered culture of human skin fibroblasts and keratinocytes through three-dimensional freeform fabrication
Multi-layered culture of human skin fibroblasts and keratinocytes through three-dimensional freeform fabrication
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DOI：
10.1016/j.biomaterials.2008.12.009
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发表时间：
2009-03-01
期刊：
影响因子：
14
通讯作者：
Yoo, Seung-Schik
中科院分区：
文献类型：
--
作者：
Lee, Wonhye;Debasitis, Jason Cushing;Yoo, Seung-Schik
我们提出了一种方法来创建由人皮肤成纤维细胞和角质形成细胞组成的模拟皮肤层的多层工程组织复合材料。使用打印胶原水凝胶前体、成纤维细胞和角质形成细胞的机器人平台实施基于油直接细胞分配的三维（3D）自由成形制造（FF）技术。通过用雾化的碳酸氢钠水溶液涂覆层来交联含细胞胶原的印刷层。在平面组织培养鱼上以逐层的方式重复该过程，得到内部成纤维细胞和外部角质形成细胞的两个不同的细胞层。为了证明在非平面表面上打印和培养多层细胞-水凝胶复合物的能力，以用于包括皮肤创伤修复的潜在应用，在具有3D表面轮廓的聚（二甲基硅氧烷）（PDMS）模具上测试该技术作为目标基底。在平面和非平面表面上均观察到每个细胞层的高度存活增殖。我们的研究结果表明，器官型皮肤组织培养是可行的，使用按需细胞打印技术与未来的潜在应用，在创建皮肤移植物定制的伤口形状或人工组织测定疾病建模和药物测试。(C)2008爱思唯尔有限公司保留所有权利。
We present a method to create multi-layered engineered tissue composites consisting of human skin fibroblasts and keratinocytes which mimic skin layers. Three-dimensional (3D) freeform fabrication (FF) technique, based oil direct cell dispensing, was implemented using a robotic platform that prints collagen hydrogel precursor, fibroblasts and keratinocytes. A printed layer of cell-containing collagen was crosslinked by coating the layer with nebulized aqueous sodium bicarbonate. The process was repeated in layer-by-layer fashion oil a planar tissue Culture fish, resulting ill two distinct cell layers of inner fibroblasts and outer keratinocytes. it order to demonstrate the ability to print and Culture multi-layer cell-hydrogel composites oil a non-planar surface for potential applications including skin Wound repair, the technique Was tested oil a poly(dimethylsiloxane) (PDMS) mold with 3D surface Contours as a target Substrate. Highly viable proliferation of each cell layer was observed on both planar and non-planar surfaces. Our results Suggest that organotypic skin tissue Culture is feasible using on-demand cell printing technique with future potential application in creating skin grafts tailored for Wound shape or artificial tissue assay for disease modeling and drug testing. (C) 2008 Elsevier Ltd. All rights reserved.